Evaluation of different laboratory tests for the detection of metallo- -lactamase production in Enterobacteriaceae
| Autor: | Panagiota Danai Rekatsina, Despina Hatzaki, Diamantis Plachouras, Helen Giamarellou, Maria Souli, Irene Galani |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Pharmacology
Microbiology (medical) Imipenem biology Clinical Laboratory Techniques Klebsiella pneumoniae Providencia stuartii Ceftazidime Microbial Sensitivity Tests Enterobacter Acinetobacter bacterial infections and mycoses biology.organism_classification Gene Expression Regulation Enzymologic beta-Lactamases Citrobacter freundii Microbiology Infectious Diseases Enterobacteriaceae polycyclic compounds medicine Humans Pharmacology (medical) Etest medicine.drug |
| Zdroj: | Journal of Antimicrobial Chemotherapy. 61:548-553 |
| ISSN: | 1460-2091 0305-7453 |
| DOI: | 10.1093/jac/dkm535 |
| Popis: | Received 17 November 2007; returned 28 November 2007; revised 5 December 2007; accepted 11 December 2007 Objectives: Clinical isolates of Klebsiella pneumoniae (91), Escherichia coli (49), Enterobacter spp. (27), Proteus mirabilis (17), Citrobacter freundii (2), Providencia stuartii (3) and Serratia spp. (5), with various MICs of imipenem, were examined for production of metallo-b-lactamases (MBLs) with different phenotypic laboratory tests that have been previously published to detect MBLs in Pseudomonas aeruginosa and Acinetobacter spp. Methods: A total of 194 (95 MBL-positive and 99 MBL-negative) clinical isolates with imipenem MICs � 0.25 to >256 mg/L were examined. All isolates were evaluated by the double-disc synergy test (DDST), the combination disc test (CDT), the MBL Etest and the modified Hodge test. The presence of blaVIM and blaIMP genes was evaluated by in situ hybridization with specific probes and was certified by PCR. Results :I n 30blaVIM-positive isolates that exhibited MICs of imipenem � 4 mg/L, MBL Etest could not be evaluated. CDT with ceftazidime and 1900 or 750 mg of EDTA, and DDST after applying an imipenem disc 10 mm apart from a disc containing 1900 mg of EDTA, showed the highest sensitivity (97.9% to 100%) and specificity (87.9% to 96%) rates among the analysed procedures. CDT with imipenem and 1900 mg of EDTA exhibited a sensitivity of 94.7% and showed very good specificity (98%). Conclusions: The CDT with imipenem/imipenem10.5 M EDTA or ceftazidime/ceftazidime10.2 M EDTA and the DDST with imipenem 10 mm apart from EDTA are the most effective methods for the detection of MBLs in Enterobacteriaceae. |
| Databáze: | OpenAIRE |
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