Time-dependent cytokine and chemokine changes in mouse cerebral cortex following a mild traumatic brain injury
| Autor: | Nigel H. Greig, Barry J. Hoffer, Edward J. Goetzl, Chaim G. Pick, Hanuma Kumar Karnati, David Tweedie, Roger J. Mullins, Dimitrios Kapogiannis |
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| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Chemokine Pathology Vascular Endothelial Growth Factor B Mouse medicine.medical_treatment Cell Cycle Proteins Mice 0302 clinical medicine Immunology and Inflammation Gene Regulatory Networks Biology (General) Cerebral Cortex biology General Neuroscience Head injury General Medicine Extracellular vesicle Cytokine Cytokines Medicine medicine.symptom Chemokines protein marker Research Article medicine.medical_specialty Traumatic brain injury QH301-705.5 Science Inflammation GPI-Linked Proteins General Biochemistry Genetics and Molecular Biology 03 medical and health sciences Extracellular Vesicles mild traumatic brain injury medicine Animals Pathological CXCL16 Brain Concussion General Immunology and Microbiology business.industry Chemokine CXCL16 medicine.disease Disease Models Animal 030104 developmental biology Gene Ontology inflammation Brain Injuries biology.protein business Transcriptome 030217 neurology & neurosurgery Biomarkers Neuroscience |
| Zdroj: | eLife, Vol 9 (2020) eLife |
| Popis: | Traumatic brain injury (TBI) is a serious global health problem, many individuals live with TBI-related neurological dysfunction. A lack of biomarkers of TBI has impeded medication development. To identify new potential biomarkers, we time-dependently evaluated mouse brain tissue and neuronally derived plasma extracellular vesicle proteins in a mild model of TBI with parallels to concussive head injury. Mice (CD-1, 30–40 g) received a sham procedure or 30 g weight-drop and were euthanized 8, 24, 48, 72, 96 hr, 7, 14 and 30 days later. We quantified ipsilateral cortical proteins, many of which differed from sham by 8 hours post-mTBI, particularly GAS-1 and VEGF-B were increased while CXCL16 reduced, 23 proteins changed in 4 or more of the time points. Gene ontology pathways mapped from altered proteins over time related to pathological and physiological processes. Validation of proteins identified in this study may provide utility as treatment response biomarkers. |
| Databáze: | OpenAIRE |
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