Comparative proteomic analysis of Aedes aegypti larval midgut after intoxication with Cry11Aa toxin from Bacillus thuringiensis

Autor: Juan Luis Jurat-Fuentes, Angeles Cancino-Rodezno, Amy E. Evans, Sergio Encarnación, Luis Lozano, Cris Oppert, Mario Soberón, Sarjeet S. Gill, Humberto Lanz-Mendoza, Julieta I. Castro, Alejandra Bravo
Přispěvatelé: Vontas, John
Rok vydání: 2012
Předmět:
0106 biological sciences
Proteomics
Insecta
Applied Microbiology
lcsh:Medicine
medicine.disease_cause
Toxicology
01 natural sciences
Biochemistry
Analytical Chemistry
Hemolysin Proteins
RNA interference
Aedes
Bacillus thuringiensis
lcsh:Science
0303 health sciences
Multidisciplinary
biology
Agriculture
Hsp90
Nucleic acids
Host-Pathogen Interaction
Insects
Chemistry
Infectious Diseases
Larva
Insect Proteins
Research Article
Biotechnology
General Science & Technology
Protein subunit
Toxic Agents
Bacterial Toxins
Microbiology
03 medical and health sciences
Bacterial Proteins
Heat shock protein
medicine
Genetics
Animals
Heat shock
Biology
030304 developmental biology
Bacillus thuringiensis Toxins
Toxin
lcsh:R
fungi
biology.organism_classification
Endotoxins
010602 entomology
Emerging Infectious Diseases
biology.protein
RNA
lcsh:Q
Pest Control
Zoology
Entomology
Zdroj: PloS one, vol 7, iss 5
PLoS ONE, Vol 7, Iss 5, p e37034 (2012)
PLoS ONE
Popis: Cry toxins produced by Bacillus thuringiensis bacteria are environmentally safe alternatives to control insect pests. They are pore-forming toxins that specifically affect cell permeability and cellular integrity of insect-midgut cells. In this work we analyzed the defensive response of Aedes aegypti larva to Cry11Aa toxin intoxication by proteomic and functional genomic analyses. Two dimensional differential in-gel electrophoresis (2D-DIGE) was utilized to analyze proteomic differences among A. aegypti larvae intoxicated with different doses of Cry11Aa toxin compared to a buffer treatment. Spots with significant differential expression (p
Databáze: OpenAIRE
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