Evaluation of a fibrin-based skin substitute prepared in a defined keratinocyte medium
| Autor: | Franc Planinsek, Miomir Knezevic, Zoran M. Arnez, Metka Krasna, Matjaz Jeras |
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| Přispěvatelé: | M., Krasna, F., Planinsek, M., Knezevic, Arnez, ZORAN MARIJ, M., Jeras |
| Jazyk: | angličtina |
| Rok vydání: | 2005 |
| Předmět: |
Keratinocytes
Cells Drug Evaluation Preclinical Pharmaceutical Science Tetrazolium Salts Human skin Fibrin Tissue Adhesive Aprotinin Cell Proliferation Cultured Drug Evaluation Skin Fibrin Microbiology medicine Humans MTT assay Clonogenic assay Fibrin glue Cells Cultured Skin Artificial biology Chemistry Cell growth Molecular biology medicine.anatomical_structure Cell culture biology.protein Cell Keratinocyte |
| Popis: | The purpose of this study was to evaluate the influence of fibrin glue and aprotinin on the growth of adult human skin keratinocytes in defined serum-free conditions. The keratinocytes were cultured on cell culture plastics and on a fibrin matrix prepared from fibrin glue. The cell growth was measured by MTT assay, while the growth of clonogenic keratinocytes was evaluated by colony assay and expressed as colony-forming efficiency (CFE). The clonogenic potential of keratinocytes released from subconfluent and confluent cultures grown on fibrin glue was also studied by the colony assay. In comparison to a plastic culture surface the fibrin glue had significantly (P0.05) increased the clonogenic potential of keratinocytes, as well as enhanced their growth. Keratinocytes released from subconfluent cultures grown on fibrin glue attained a significantly (P0.05) higher percentage of clonogenic cells than their confluent parallels. At 75, 150, 300 and 450 KIU/ml aprotinin did not influence the growth of keratinocytes (P0.2). A fibrin-based skin substitute produced in the defined keratinocyte medium could be safely used to treat a number of skin defects. |
| Databáze: | OpenAIRE |
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