Polarized 86Rb+ effluxes in primary cultures of rabbit kidney proximal cells: role of calcium and hypotonicity
| Autor: | Philippe Poujeol, S. Le Maout, N. Koechlin, Michel Tauc |
|---|---|
| Přispěvatelé: | Physiologie cellulaire et moléculaire des systèmes intégrés (PCMSI), Université Nice Sophia Antipolis (... - 2019) (UNS), COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-COMUE Université Côte d'Azur (2015-2019) (COMUE UCA)-Centre National de la Recherche Scientifique (CNRS) |
| Jazyk: | angličtina |
| Rok vydání: | 1990 |
| Předmět: |
Male
Cell Membrane Permeability Charybdotoxin [SDV]Life Sciences [q-bio] Barium Compounds Biophysics Ionophore Fluorescence spectrometry chemistry.chemical_element Fluorescent Antibody Technique Calcium Apamin Biochemistry Kidney Tubules Proximal 03 medical and health sciences chemistry.chemical_compound Chlorides Cyclic AMP Animals Virulence Factors Bordetella Ion transporter Cells Cultured ComputingMilieux_MISCELLANEOUS 030304 developmental biology 0303 health sciences Tetraethylammonium Ionomycin 030302 biochemistry & molecular biology Cell Membrane Cell Biology Hydrogen-Ion Concentration Tetraethylammonium Compounds Microscopy Electron chemistry Hypotonic Solutions Pertussis Toxin Barium Potassium Rabbits Rubidium Radioisotopes |
| Zdroj: | Biochimica et Biophysica Acta:Biomembranes Biochimica et Biophysica Acta:Biomembranes, Elsevier, 1990, 1026 (1), pp.29-39. ⟨10.1016/0005-2736(90)90328-l⟩ |
| ISSN: | 0005-2736 1879-2642 |
| DOI: | 10.1016/0005-2736(90)90328-l⟩ |
| Popis: | Isolated proximal cells from rabbit kidney were seeded on collagen-coated permeable supports. After 8 days, the cultured cells became organized as a confluent monolayer. The proximal origin of the monolayer was confirmed by enzymatic, immunological, electrical and electron microscopical studies. The epithelia exhibited a morphological polarity that allowed for measurements of effluxes across the apical or the basolateral membranes. 86Rb was used as an isotopic tracer to indicate potassium movements. The 86Rb+ efflux across the basolateral face was 1.93-times that across the apical face, and both effluxes were pH dependent. Apical and basolateral 86Rb+ effluxes increased when the Ca2+ ionophore ionomycin (3 μM) was applied and when monolayers were exposed to a hypotonic medium. A pharmacological study revealed that BaCl2 (5 mM), tetraethylammonium (TEA, 20 mM) and Leiurus quinquestriatus hebraeus scorpion venom (from which charybdotoxin is extracted) abolished both ionomycin and hypotonically-stimulated effluxes, whereas apamin had no significant effect on the hypotonically-stimulated 86Rb+ efflux. This stimulated efflux was also abolished when monolayers were preincubated with pertussis toxin, but did not decrease in a Ca2+-free medium. |
| Databáze: | OpenAIRE |
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