Use of microreactors and freeze-drying in the manufacturing process of chitosan coated PCL nanoparticles
| Autor: | Antonello Barresi, Gladys E. Granero, Renée Onnainty, Tereza Zelenková, Davide Fissore |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
Sucrose
Materials science Drug Compounding Polyesters Pharmaceutical Science Nanoparticle 02 engineering and technology Poloxamer INGENIERÍAS Y TECNOLOGÍAS 030226 pharmacology & pharmacy 03 medical and health sciences chemistry.chemical_compound solvent displacement 0302 clinical medicine Drug Stability Ciprofloxacin purl.org/becyt/ford/2.10 [https] Acetone Zeta potential NANOPARTICLES confined impinging jets mixer CONFINED IMPINGING JETS MIXER CHITOSAN Nanotecnología Chitosan Drug Carriers nanotechnology NANOTECHNOLOGY Vortex mixer 021001 nanoscience & nanotechnology Nano-materiales MULTI-INLET VORTEX MIXER Anti-Bacterial Agents multi-inlet vortex mixer Solvent Freeze Drying chemistry Chemical engineering purl.org/becyt/ford/2 [https] freeze-drying Polycaprolactone Nanoparticles Particle size chitosan Microreactor 0210 nano-technology FREEZE-DRYING SOLVENT DISPLACEMENT |
| Zdroj: | CONICET Digital (CONICET) Consejo Nacional de Investigaciones Científicas y Técnicas instacron:CONICET |
| Popis: | This paper is focused on the synthesis of chitosan-coated polycaprolactone nanoparticles in microreactors and on the freeze-drying of the nanosuspension, to separate the particles from the liquid phase. Nanoparticles were produced in the confined impinging jets mixer (CIJM) and in the multi-inlet vortex mixer (MIVM), using the solvent displacement method, with acetone or tert-butanol (TBA) as polymer solvent. The study was initially carried out considering a feed flow rate of 80 ml min−1: using acetone, the mean particle size was lower (163 ± 7 nm) and the Zeta potential was higher (31.4 ± 37 mV) with the MIVM, with respect to the CIJM (265 ± 31 nm and 9.8 ± 2.4 mV, respectively). Slightly larger particles were obtained using TBA in the MIVM (mean diameter: 221 ± 44 nm): in this case it is no longer required removing the solvent before the freeze-drying stage. The effect of the liquid flow rate was then investigated, confirming that the best results were obtained at 80 ml min−1. With respect to the freeze-drying process, the effect of lyoprotectants and of steric stabilizers on particle stability was investigated. Best results were obtained with 5% sucrose and 2.5% Poloxamer 388 (mean diameter: 306 ± 8 nm); in all cases Zeta potential remained positive and larger than +30 mV. Preliminary results about the encapsulation of a test drug, ciprofloxacin, are also shown and discussed. Fil: Zelenková, Tereza. Politecnico di Torino; Italia. Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino; Italia Fil: Onnainty, Renée. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Granero, Gladys Ester. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Córdoba. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica. Universidad Nacional de Córdoba. Facultad de Ciencias Químicas. Unidad de Investigación y Desarrollo en Tecnología Farmacéutica; Argentina Fil: Barresi, Antonello A.. Politecnico di Torino; Italia. Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino; Italia Fil: Fissore, Davide. Politecnico di Torino; Italia. Dipartimento di Scienza Applicata e Tecnologia, Politecnico di Torino, Corso Duca degli Abruzzi 24, 10129 Torino; Italia |
| Databáze: | OpenAIRE |
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