Temperature- and Salinity-Decoupled Overproduction of Hydroxyectoine by Chromohalobacter salexigens
| Autor: | Joaquín J. Nieto, Montserrat Argandoña, Fernando Iglesias-Guerra, Carmen Vargas, Javier Rodríguez-Moya |
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| Přispěvatelé: | Universidad de Sevilla. Departamento de Microbiología y Parasitología, Universidad de Sevilla. Departamento de Química Orgánica y Farmacéutica |
| Rok vydání: | 2013 |
| Předmět: |
Salinity
Low salinity Gene Dosage Biology Ectoine Applied Microbiology and Biotechnology Metabolic engineering Chromohalobacter salexigens chemistry.chemical_compound Plasmid Chromohalobacter Genetic backgrounds Overproduction Gene Genetics Ecology Temperature Coexpressed Amino Acids Diamino biology.organism_classification Metabolic Engineering Biochemistry chemistry Metabolic Networks and Pathways Plasmids Food Science Biotechnology |
| Zdroj: | idUS. Depósito de Investigación de la Universidad de Sevilla instname |
| ISSN: | 1098-5336 0099-2240 |
| DOI: | 10.1128/aem.02774-12 |
| Popis: | Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine ( ectABC ) and hydroxyectoine ( ectD ) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines. |
| Databáze: | OpenAIRE |
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