Interleukin-8 enhances myocilin expression, Akt-FoxO3 signaling and myogenic differentiation in rat skeletal muscle cells
| Autor: | Tomasz Domoradzki, Marta Milewska, Magdalena Hulanicka, Małgorzata Gajewska, Katarzyna Grzelkowska-Kowalczyk, Alicja Majewska, Anna Ciecierska, Maciej Błaszczyk |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Physiology Ubiquitin-Protein Ligases Clinical Biochemistry Muscle Fibers Skeletal Adipose tissue Muscle Proteins Muscle Development Muscle hypertrophy Myoblasts Tripartite Motif Proteins 03 medical and health sciences 0302 clinical medicine medicine Myocyte Animals Insulin-Like Growth Factor I Eye Proteins Muscle Skeletal Protein kinase B Myocilin Glycoproteins Chemistry Forkhead Box Protein O3 Interleukin-8 Skeletal muscle Cell Differentiation Cell Biology Cell biology Rats Cytoskeletal Proteins 030104 developmental biology medicine.anatomical_structure 030220 oncology & carcinogenesis FOXO3 Phosphorylation Proto-Oncogene Proteins c-akt Signal Transduction |
| Zdroj: | Journal of cellular physiology. 234(11) |
| ISSN: | 1097-4652 |
| Popis: | Interleukin (IL)-8 is released both in visceral adipose tissue and in contracting skeletal muscles. In this study, we examined cellular pathways associated with muscle hypertrophy, chosen on the basis of microRNA profiling, in differentiating rat primary skeletal muscle cells (RSkMC) treated with IL-8 (1 ng/ml) for 11 days. IL-8 increased myocilin expression, Akt phosphorylation, FoxO3 dispersion throughout the cytoplasm, and reduced FoxO3 level. IL-8 decreased the expression of atrogin and MuRF1 and increased myotube length and diameter. We concluded that IL-8 present in extracellular environment of myoblasts induced to differentiation stimulates expression of myocilin, a protein important for skeletal muscle hypertrophy. This phenomenon was associated with: (a) activation of myogenic transcription, (b) increased phosphorylation and activation of PKB/Akt, leading to (c) cytoplasm distribution and degradation of a transcription factor FoxO3, (d) decreased expression of gene markers of proteolysis, atrogin and Murf1, and (e) increased myotube length and diameter. In this regard, IL-8 affects skeletal muscle cells similarly to IGF-I and can be considered as a potent anticatabolic factor for skeletal muscle. |
| Databáze: | OpenAIRE |
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