Differential Effects on Light Chain Amyloid Formation Depend on Mutations and Type of Glycosaminoglycans
| Autor: | Jared A. Hammernik, Marta Marin-Argany, Luis M. Blancas-Mejia, Marina Ramirez-Alvarado |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Amyloid
viruses education Mutation Missense Biophysics Amyloidogenic Proteins Immunoglobulin domain Fibril Protein Aggregation Pathological Biochemistry Extracellular matrix Glycosaminoglycan Immunoglobulin kappa-Chains chemistry.chemical_compound hemic and lymphatic diseases mental disorders medicine Humans Molecular Biology integumentary system Amyloidosis Chondroitin Sulfates Cell Biology Heparan sulfate medicine.disease carbohydrates (lipids) Amino Acid Substitution chemistry Protein Structure and Folding Protein folding Heparitin Sulfate |
| Zdroj: | Journal of Biological Chemistry. 290:4953-4965 |
| ISSN: | 0021-9258 |
| DOI: | 10.1074/jbc.m114.615401 |
| Popis: | Amyloid light chain (AL) amyloidosis is a protein misfolding disease where immunoglobulin light chains sample partially folded states that lead to misfolding and amyloid formation, resulting in organ dysfunction and death. In vivo, amyloid deposits are found in the extracellular space and involve a variety of accessory molecules, such as glycosaminoglycans, one of the main components of the extracellular matrix. Glycosaminoglycans are a group of negatively charged heteropolysaccharides composed of repeating disaccharide units. In this study, we investigated the effect of glycosaminoglycans on the kinetics of amyloid fibril formation of three AL cardiac amyloidosis light chains. These proteins have similar thermodynamic stability but exhibit different kinetics of fibril formation. We also studied single restorative and reciprocal mutants and wild type germ line control protein. We found that the type of glycosaminoglycan has a different effect on the kinetics of fibril formation, and this effect seems to be associated with the natural propensity of each AL protein to form fibrils. Heparan sulfate accelerated AL-12, AL-09, κI Y87H, and AL-103 H92D fibril formation; delayed fibril formation for AL-103; and did not promote any fibril formation for AL-12 R65S, AL-103 delP95aIns, or κI O18/O8. Chondroitin sulfate A, on the other hand, showed a strong fibril formation inhibition for all proteins. We propose that heparan sulfate facilitates the formation of transient amyloidogenic conformations of AL light chains, thereby promoting amyloid formation, whereas chondroitin sulfate A kinetically traps partially unfolded intermediates, and further fibril elongation into fibrils is inhibited, resulting in formation/accumulation of oligomeric/protofibrillar aggregates. |
| Databáze: | OpenAIRE |
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