Recombinant GRA4 or ROP2 Protein Combined with Alum or thegra4Gene Provides Partial Protection in Chronic Murine Models of Toxoplasmosis
| Autor: | Adolfo R. de Roodt, Alicia Supanitsky, Valentina Martin, Eduardo A. Guarnera, Silvana Litwin, Tamara Tanos, Pablo Christian Echeverria, Sergio O. Angel |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Microbiology (medical)
medicine.medical_treatment Clinical Biochemistry Immunology Protozoan Proteins Antibodies Protozoan Antibodies and Mediators of Immunity Enzyme-Linked Immunosorbent Assay Proteínas Recombinantes Immunoglobulin G Microbiology Interferon-gamma Mice chemistry.chemical_compound Vacunas de ADN Adjuvants Immunologic parasitic diseases Vaccines DNA medicine Animals Immunology and Allergy Interferon gamma Modelos Animales de Enfermedad biology Cysts Alum Vaccination Vacunación Brain Membrane Proteins Toxoplasma gondii medicine.disease biology.organism_classification Virology Recombinant Proteins Toxoplasmosis Disease Models Animal Toxoplasmosis Animal Immunization chemistry biology.protein Alum Compounds Interleukin-4 Toxoplasma Adjuvant medicine.drug |
| Zdroj: | Clinical and Diagnostic Laboratory Immunology, 2004, 11(4), 704-710. Sistema de Gestión del Conocimiento ANLIS MALBRÁN Administración Nacional de Laboratorios e Institutos de Salud "Dr. Carlos G. Malbrán" instacron:ANLIS |
| ISSN: | 1556-679X 1556-6811 |
| DOI: | 10.1128/cdli.11.4.704-710.2004 |
| Popis: | The efficacy of vaccination with Toxoplasma gondii recombinant GRA4 (rGRA4) and ROP2 (rRPO2) proteins and a mix of both combined with alum were evaluated in C57BL/6 and C3H mice. In C57BL/6 mice, rGRA4 and rGRA4-rROP2 immunizations generated similar levels of immunoglobulin G1 (IgG1) and IgG2a isotypes against GRA4, whereas immunizations with rROP2 and the mix induced a predominant IgG1 production against ROP2. All groups of C3H vaccinated mice exhibited higher levels of IgG1 than IgG2a. rGRA4-stimulated splenocytes from vaccinated mice produced primarily gamma interferon while those stimulated with rROP2 produced interleukin-4. Challenge of rGRA4- or rGRA4-rROP2-vaccinated mice from both strains with ME49 cysts resulted in fewer brain cysts than the controls, whereas vaccination with rROP2 alone only conferred protection to C3H mice. Immunization with a plasmid carrying the entire open reading frame of GRA4 showed a protective level similar to that of rGRA4 combined with alum. These results suggest that GRA4 can be a good candidate for a multiantigen anti-T. gondii vaccine based on the use of alum as an adjuvant. Fil: Martin, Valentina. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Supanitsky, Alicia. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Echeverria, Pablo C. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Litwin, Silvana. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina. Fil: Tanos, Tamara. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: de Roodt, Adolfo R. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Producción de Biológicos; Argentina. Fil: Guarnera, Eduardo. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. Fil: Angel, Sergio O. ANLIS Dr.C.G.Malbrán. Instituto Nacional de Enfermedades Infecciosas. Departamento de Parasitología; Argentina. |
| Databáze: | OpenAIRE |
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