Cerebral DARPP‐32 expression after methylphenidate administration in young and adult rats
| Autor: | Cecília G. Inácio, Marco Aurélio Romano-Silva, Bruno R. Souza, Márcio R. Martins, M. V. Gomez, Samira S. Valvassori, Daniela V.F. Rosa, Eliane C. Soares, João Quevedo, Karin Martins Gomes, Renan P. Souza, Gislaine Z. Réus |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Aging
Dopamine and cAMP-Regulated Phosphoprotein 32 medicine.medical_specialty Dopamine Hippocampus Striatum Hippocampal formation Drug Administration Schedule Developmental Neuroscience Internal medicine medicine Animals Prefrontal cortex Dopamine transporter Cerebral Cortex biology Methylphenidate Dopaminergic Brain Rats Up-Regulation Endocrinology Attention Deficit Disorder with Hyperactivity biology.protein Central Nervous System Stimulants Psychology Neuroscience Developmental Biology medicine.drug |
| Zdroj: | International Journal of Developmental Neuroscience. 27:1-7 |
| ISSN: | 1873-474X 0736-5748 |
| Popis: | Dopamine may alter the phosphorylation state of DARPP-32 that plays a central role in the dopaminergic neurons biology. Studies have shown that DARPP-32/protein phosphatase 1 cascade is a major target for psychostimulants drugs. Methylphenidate is a psychostimulant that acts blocking the dopamine transporter has been used as an effective treatment for Attention Deficit Hyperactivity Disorder. We investigated if methylphenidate could alter DARPP-32 expression in five brain regions (striatum, hippocampus, prefrontal cortex, cortex and cerebellum) in young and adult rats. Our results showed that methylphenidate treatment is able to alter DARPP-32 expression in rat brain. Acute methylphenidate treatment has reduced hippocampal DARPP-32 protein levels in old rats, while chronic methylphenidate treatment has decreased them in old rat hippocampus and young rat cerebellum. It was found an increased cortical expression after chronic methylphenidate administration in old rats. Our results provide the first experimental demonstration that methylphenidate induces changes in total DARPP-32 expression that are posology- and age-related in some rat brain areas, although further studies are needed to shed more light on the mechanisms behind these findings. |
| Databáze: | OpenAIRE |
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