Modification of the coding sequence enhances plant expression of insect control protein genes
| Autor: | Roy L. Fuchs, Sylvia L. McPherson, David A. Fischhoff, Frederick J. Perlak, Duff A. Dean |
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| Rok vydání: | 1991 |
| Předmět: |
Translational efficiency
Transcription Genetic Nicotiana tabacum Bacterial Toxins DNA Mutational Analysis Molecular Sequence Data Bacillus thuringiensis Regulatory Sequences Nucleic Acid Hemolysin Proteins Bacterial Proteins Gene expression Tobacco RNA Messenger Cloning Molecular Codon Gene Regulation of gene expression Genetics Multidisciplinary biology Bacillus thuringiensis Toxins Base Sequence fungi Nucleic acid sequence food and beverages Plants biology.organism_classification Molecular biology Endotoxins Plants Toxic Gene Expression Regulation Regulatory sequence Protein Biosynthesis Genetic Engineering Research Article |
| Zdroj: | Proceedings of the National Academy of Sciences of the United States of America. 88(8) |
| ISSN: | 0027-8424 |
| Popis: | Increased expression of the insect control protein genes of Bacillus thuringiensis in plants has been critical to the development of genetically improved plants with agronomically acceptable levels of insect resistance. The expression of the cryIA(b) gene was compared to partially modified (3% nucleotide difference) and to fully modified (21% nucleotide difference) cryIA(b) and cryIA(c) genes in tobacco and tomato. The modified genes increased the frequency of plants that produced the proteins at quantities sufficient to control insects and dramatically increased the levels of these proteins. Among the most highly expressing transformed plants for each gene, the plants with the partially modified cryIA(b) gene had a 10-fold higher level of insect control protein and plants with the fully modified cryIA(b) had a 100-fold higher level of CryIA(b) protein compared with the wild-type gene. Similar results were obtained with the fully modified cryIA(c) gene in plants. Specific sequences of the partially modified cryIA(b) gene were analyzed for their ability to affect cryIA(b) gene expression in tobacco. The DNA sequence of a single region was identified as important to the improvement of plant expression of the cryIA(b) gene. The increased levels of cryIA(b) mRNA were not directly proportional to the increased levels of CryIA(b) protein in plants transformed with the modified cryIA(b) genes, indicating that the nucleotide sequence of these genes had an effect in improving their translational efficiency in plants. |
| Databáze: | OpenAIRE |
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