Prevalence and molecular epidemiology of acquired AmpC β-lactamases and carbapenemases in Enterobacteriaceae isolates from 35 hospitals in Spain

Autor: Germán Bou, Antonio Oliver, Jesús Agüero, Jesús Rodríguez-Baño, Juan José González-López, M. C. Conejo, Laura Zamorano, Luis Martínez-Martínez, Belén Aracil, Elisenda Miró, Ana Fernández, Ferran Navarro, Jesús Oteo, Noelia Lara, Álvaro Pascual, María Nieves Larrosa
Rok vydání: 2012
Předmět:
Imipenem
sequence analysis
cephalosporin
Cephalosporin
Polymerase Chain Reaction
molecular epidemiology
carbapenemase
amikacin
Genotype
Prevalence
Prospective Studies
amoxicillin plus clavulanic acid
Cross Infection
Molecular Epidemiology
biology
Enterobacteriaceae Infections
article
General Medicine
Enterobacteriaceae
Hospitals
protein CMY 2
Anti-Bacterial Agents
Electrophoresis
Gel
Pulsed-Field
unclassified drug
Infectious Diseases
priority journal
bla gene
bacterial gene
Plasmids
prospective study
medicine.drug
DNA
Bacterial
Microbiology (medical)
phenotype
medicine.drug_class
prevalence
tobramycin
Microbial Sensitivity Tests
beta lactamase AmpC
gentamicin
minimum inhibitory concentration
beta-Lactamases
Microbiology
Minimum inhibitory concentration
ciprofloxacin
Escherichia coli
medicine
Pulsed-field gel electrophoresis
Humans
controlled study
Proteus mirabilis
nonhuman
Molecular epidemiology
bacterial enzyme
bacterium isolate
protein DHA 1
nucleotide sequence
Sequence Analysis
DNA
biochemical phenomena
metabolism
and nutrition
bacterial strain
bacterial infections and mycoses
biology.organism_classification
cotrimoxazole
antibiotic sensitivity
Spain
Zdroj: EUROPEAN JOURNAL OF CLINICAL MICROBIOLOGY & INFECTIOUS DISEASES
r-IIB SANT PAU. Repositorio Institucional de Producción Científica del Instituto de Investigación Biomédica Sant Pau
instname
ISSN: 1435-4373
0934-9723
DOI: 10.1007/s10096-012-1737-0
Popis: The purpose of this investigation was to determine the prevalence of plasmid-mediated AmpC (pAmpC) and carbapenemases in Enterobacteriaceae collected from 35 hospitals in Spain and to establish their epidemiological relationships. We conducted a prospective multi-centre study on pAmpC- or carbapenemase-producing Enterobacteriaceae isolates from clinical samples collected from February to July 2009. The strains suspected to carry pAmpC were resistant or showed intermediate susceptibility to co-amoxiclav and second- or third-generation cephalosporins. Strains suspected to carry a carbapenemase were selected because they showed a minimum inhibitory concentration (MIC) to imipenem >1 mg/L. Polymerase chain reaction (PCR) and a sequencing strategy were used to characterise the enzymes. The clonal relationships between isolates was analysed by pulsed field gel electrophoresis (PFGE). Among 100,132 Enterobacteriaceae isolates collected, 1,654 were compatible with the production of pAmpC or carbapenemases. We found a prevalence of 0.64 % of pAmpC (n = 635) and 0.04 % of carbapenemases (n = 43). The most prevalent pAmpC enzymes were CMY-type (78.3 %), DHA-type (19.5 %), ACC-type (1.6 %) and FOX-type (0.6 %). The CMY-type was the most frequent in Escherichia coli and Proteus mirabilis species, whereas the DHA-type was mainly found in Klebsiella spp. The enzymes involved in carbapenem resistance were VIM-1, IMP-22 and the new IMP-28. Nine new bla genes were described: bla CMY-54, bla CMY-55, bla CMY-56, bla CMY-57, bla CMY-96, bla DHA-6, bla DHA-7, bla FOX-8 and bla IMP-28. The prevalence of pAmpC or carbapenemases found is not negligible. The CMY-types were the predominant pAmpC, whereas the VIM or IMP enzymes were the predominant carbapenemases. Furthermore, we observed a great genetic diversity among pAmpC-producing strains and a close clonal relationship between carbapenemase-producing strains.
Databáze: OpenAIRE
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