Four paralogous Gfh factors in the extremophilic bacterium Deinococcus peraridilitoris have distinct effects on various steps of transcription
| Autor: | Andrey Kulbachinskiy, Aleksei Agapov |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0301 basic medicine
Transcription Genetic Sequence Homology Biochemistry 03 medical and health sciences chemistry.chemical_compound Bacterial Proteins Transcription (biology) RNA polymerase Intrinsic termination Homologous chromosome Deinococcus Amino Acid Sequence Polymerase RNA Cleavage 030102 biochemistry & molecular biology biology DNA-Directed RNA Polymerases Gene Expression Regulation Bacterial General Medicine biology.organism_classification Cell biology RNA Bacterial 030104 developmental biology chemistry biology.protein Nucleic Acid Conformation Bacteria |
| Zdroj: | Biochimie. 170:21-25 |
| ISSN: | 0300-9084 |
| Popis: | Gre factors are ubiquitous transcription regulators that stimulate co-transcriptional RNA cleavage by bacterial RNA polymerase (RNAP). Here, we show that the stress-resistant bacterium Deinococcus peraridilitoris encodes four Gre factor homologs, Gfh proteins, that have distinct effects on transcription by RNAP. Two of the factors, Gfh1α and Gfh2β inhibit transcription initiation, and one of them, Gfh1α can also regulate transcription elongation. We show that this factor strongly stimulates transcriptional pausing and intrinsic termination in the presence of manganese ions but has no effect on RNA cleavage. Thus, some Gfh factors encoded by Deinococci serve as lineage-specific transcription inhibitors that may play a role in stress resistance, while the functions of others remain to be discovered. |
| Databáze: | OpenAIRE |
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