Reaction of tryptophan with carbohydrates: identification and quantitative determination of novel beta-carboline alkaloids in food
| Autor: | Stefanie Diem, Markus Herderich |
|---|---|
| Rok vydání: | 2001 |
| Předmět: |
Indole test
Pictet–Spengler reaction Chromatography Chemistry Tryptophan Diastereomer General Chemistry Nuclear magnetic resonance spectroscopy Tandem mass spectrometry Mass spectrometry High-performance liquid chromatography Mass Spectrometry Maillard Reaction Alkaloids Glucose Spectrophotometry Ultraviolet General Agricultural and Biological Sciences Chromatography High Pressure Liquid Food Analysis Carbolines |
| Zdroj: | Journal of agricultural and food chemistry. 49(5) |
| ISSN: | 0021-8561 |
| Popis: | The formation of various carbohydrate-derived beta-carbolines was observed when model reactions of tryptophan with glucose were studied by means of HPLC with diode array detection, as well as by means of HPLC-MS. Isolation of these compounds and subsequent characterization by tandem mass spectrometry and NMR spectroscopy led to the identification of diastereomeric 1-(1,3,4,5-tetrahydroxypent-1-yl)-9H-pyrido[3,4-b]indoles (1a/b), 1-(1,4,5-trihydroxypent-1-yl)-9H-pyrido[3,4-b]indoles (2a/b), and E/Z isomers of 1-(1,5-dihydroxypent-3-en-1-yl)-9H-pyrido[3,4-b]indole (3a/b). HPLC-MS was used to prove the presence of these novel beta-carboline alkaloids in various food samples. In addition, quantitative determination of beta-carbolines 1a, 1b, and 2a/b in numerous products was achieved by means of HPLC with fluorometric detection. Concentrations ranged from 12 to 1922 microg/L for 1a and 1b and from 3 to 644 microg/L for 2a/b. The highest concentrations of all carbohydrate-derived beta-carbolines under study were found in ketchup, soy sauce, and fish sauce. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|