Antibiotic treatment of the hard tick Ixodes ricinus: Influence on Midichloria mitochondrii load following blood meal
| Autor: | Valentina Serra, Olivier Plantard, Chiara Bazzocchi, Claudia Pollera, Alessandra Cafiso, Davide Sassera, Camille Ninio, Nicola Ferrari |
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| Přispěvatelé: | Biologie, Epidémiologie et analyse de risque en Santé Animale (BIOEPAR), Institut National de la Recherche Agronomique (INRA), Oniris, UMR BioEpAR, PRES Université Nantes Angers Le Mans (UNAM), Transmission Vectorielle et Épidémiosurveillance de Maladies Parasitaires - EA 4688 (VECPAR), SFR CAP Santé (Champagne-Ardenne Picardie Santé), Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA)-Université de Picardie Jules Verne (UPJV)-Université de Reims Champagne-Ardenne (URCA), Department of Veterinary Science and Public Health, Università degli Studi di Milano [Milano] (UNIMI), Università degli Studi di Pavia, Partenaires INRAE |
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Nymph
Ixodes ricinus Midichloria mitochondrii Tetracycline [SDV]Life Sciences [q-bio] Population Tick Microbiology parasitic diseases medicine Animals education Symbiosis Alphaproteobacteria education.field_of_study biology Ixodes Host (biology) Ricinus fungi biology.organism_classification Blood meal Antibiotic treatment Microinjection 3. Good health Anti-Bacterial Agents Real time PCR Infectious Diseases Parasitology Insect Science Larva Female Gerbillinae medicine.drug |
| Zdroj: | Ticks and Tick-borne Diseases Ticks and Tick-borne Diseases, Elsevier, 2015, 6 (5), pp.653-657. ⟨10.1016/j.ttbdis.2015.05.011⟩ |
| ISSN: | 1877-959X |
| DOI: | 10.1016/j.ttbdis.2015.05.011⟩ |
| Popis: | International audience; Midichloria mitochondrii is the most prevalent symbiont of the hard tick Ixodes ricinus, present in 100% of eggs and adult females of wild ticks. This bacterium is intracellular, and is the only known symbiont able to invade the mitochondria of the host cells. However, the role that M. mitochondrii plays in the host metabolism has yet to be elucidated. Multiple lines of evidence indicate the possibility of transmission of this bacterium to the vertebrate host during the tick blood meal. In order to investigate the role of M. mitochondrii in the biology of the tick host, we performed an antibiotic treatment on Ixodes ricinus individuals, with the aim of reducing/eliminating the symbiont, and to potentially observe the dynamic of bacterial infection in the tick host. We microinjected engorged adult females of I. ricinus with tetracycline, and we allowed the resulting larvae to feed on gerbils treated with the same antibiotic. The amount of M. mitochondrii was evaluated at different stages of the experiment using molecular techniques. In addition we evaluated the presence/absence of the symbiont DNA in the blood of gerbils used for the larval feeding. The performed treatments did not allow to eliminate the symbiont population from the host tick, however it allowed to reduce the multiplication that occurs after the larval blood meal. These results open the way for future experiments, using different antibiotic molecules, different administration methods and antibiotic administration on subsequent tick stages, to fulfill the goal of eliminating M. mitochondrii from the host I. ricinus, a major step in our understanding of the impact of this bacterium on ticks |
| Databáze: | OpenAIRE |
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