Tempol reduces bacterial translocation after ischemia/reperfusion injury in a rat model of superior mesenteric artery occlusion
| Autor: | Gurkan Tellioglu, Nural Cevahir, Cagatay Aydin, Goksel Kocbil, Gulistan Gumrukcu, İbrahim Berber, Cigdem Yenisey, Koray Tekin |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: |
Male
Antioxidant antioxidant Neutrophils medicine.medical_treatment Myocardial Ischemia reactive oxygen metabolite Wistar rat Antioxidants Surgical oncology intestine injury Malondialdehyde Occlusion rat animal Superior mesenteric artery glutathione Intestinal Mucosa acute disease chemistry.chemical_classification malonaldehyde article superior mesenteric artery neutrophil lipid peroxidation General Medicine continuous infusion reperfusion injury enzyme activity Mesenteric Arteries myeloperoxidase Anesthesia Models Animal intestine mucosa superior mesenteric artery obstruction mesenteric artery enzymology animal experiment Ischemia Ischemia/reperfusion complication Arterial Occlusive Diseases Myocardial Reperfusion animal tissue heart muscle reperfusion Cyclic N-Oxides medicine.artery medicine spin labeling Animals bacterial translocation controlled study drug dose reduction Rats Wistar Reactive oxygen species nonhuman business.industry animal model disease model Tempol medicine.disease Rats chemistry Mesenteric ischemia drug effects amine oxide Surgery pathology Spin Labels business Reactive Oxygen Species Reperfusion injury metabolism peripheral occlusive artery disease |
| Popis: | Purpose: We investigated whether Tempol, a water-soluble antioxidant, prevents the harmful effects of superior mesenteric ischemia/reperfusion on intestinal tissues in rats. Methods: The rats were divided into three groups of 10. In group 1, the superior mesenteric artery (SMA) was isolated but not occluded, and in groups 2 and 3 the superior mesenteric artery was occluded for 60 min. After that, the clamp was removed and reperfusion began. In group 3, 5 min before the start of reperfusion, a bolus dose of 30 mg/kg Tempol was administered intravenously and continued at a dose of 30 mg/kg for 60 min. All animals were euthanized after 24 h and tissue samples were collected for analysis. Results: There was a significant increase in myeloperoxidase activity, malondialdehyde levels, and the incidence of bacterial translocation in group 2, with a decrease in glutathione levels. These parameters were found to be normalized in group 3. The intestinal mucosal injury score in group 2 was significantly higher than those in groups 1 and 3. Conclusion: Tempol prevents bacterial translocation while precluding the harmful effects of ischemia/reperfusion injury on intestinal tissues in a rat model of superior mesenteric artery occlusion. © 2009 Springer. |
| Databáze: | OpenAIRE |
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