A Cryopreservation Method for Pasteurella multocida from Wetland Samples
| Autor: | Daniel J. Shadduck, Diane R. Goldberg, Michael D. Samuel, Melody K. Moore |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
Pasteurella multocida
animal diseases Fresh Water Sensitivity and Specificity Cryopreservation Microbiology Mice chemistry.chemical_compound otorhinolaryngologic diseases Isolation techniques Animals Bioassay Ecology Evolution Behavior and Systematics Chromatography Ecology biology Inoculation Dimethyl sulfoxide food and beverages respiratory system biology.organism_classification Isolation (microbiology) chemistry Biological Assay Water Microbiology |
| Zdroj: | Journal of Wildlife Diseases. 34:182-185 |
| ISSN: | 0090-3558 |
| Popis: | A cryopreservation method and improved isolation techniques for detection of Pasteurella multocida from wetland samples were developed. Wetland water samples were collected in the field, diluted in dimethyl sulfoxide (DMSO, final concentration 10%), and frozen at -180 C in a liquid nitrogen vapor shipper. Frozen samples were transported to the laboratory where they were subsequently thawed and processed in Pasteurella multocida selective broth (PMSB) to isolate P. multocida. This method allowed for consistent isolation of 2 to 18 organisms/ml from water seeded with known concentrations of P. multocida. The method compared favorably with the standard mouse inoculation method and allowed for preservation of the samples until they could be processed in the laboratory. |
| Databáze: | OpenAIRE |
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