Study on antidepressant mechanism of Radix Bupleuri–Radix Paeoniae Alba herb pair by metabonomics combined with 1H nuclear magnetic resonance and ultra-high-performance liquid chromatography-tandem mass spectrometry detection technology

Autor: Tian Li, Guanhua Du, Wenxia Gong, Xue-Mei Qin, Shiwei Zhu, Zhou Yuzhi
Rok vydání: 2021
Předmět:
Magnetic Resonance Spectroscopy
Proton Magnetic Resonance Spectroscopy
Pharmaceutical Science
Traditional Chinese medicine
Paeonia
Plant Roots
Rats
Sprague-Dawley
03 medical and health sciences
0302 clinical medicine
Nuclear magnetic resonance
Tandem Mass Spectrometry
Liquid chromatography–mass spectrometry
Herb pair
Animals
Metabolomics
Radix
Medicine
Chinese Traditional
Chromatography
High Pressure Liquid
030304 developmental biology
Pharmacology
Radix bupleuri
Depressive Disorder
0303 health sciences
Behavior
Animal
Depression
Chemistry
Magnetic Resonance Imaging
Antidepressive Agents
Bupleurum
Liver
Metabolome
Antidepressant
Ultra high performance
Metabolic Networks and Pathways
Stress
Psychological
030217 neurology & neurosurgery
Drugs
Chinese Herbal
Phytotherapy
Behavioural despair test
Zdroj: Journal of Pharmacy and Pharmacology. 73:1262-1273
ISSN: 2042-7158
0022-3573
DOI: 10.1093/jpp/rgab061
Popis: Objectives Radix Bupleuri–Radix Paeoniae Alba (BP), a traditional Chinese medicine herb pair, has treated depression by coordinating the liver in Chinese classical medicine books and modern research. This study aims to verify the antidepressant effect of BP by behavioural examination, and reveal the underlying antidepressant mechanisms of BP. Methods The antidepressant effects in chronic unpredictable mild stress (CUMS) of BP were observed by behavioural indicators and 1H nuclear magnetic resonance (1H-NMR) and ultra-high-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) metabonomics techniques combined with the related analysis platforms. Key findings BP could significantly improve the depressive behaviour of CUMS rats. Compared with the model group, body weight (P < 0.05), the number of crossing (P < 0.001) and rearing (P < 0.01) and sucrose preference rate (P < 0.01) were significantly enhanced, and the immobility time was shortened in the forced swimming test (P < 0.001) of the BP group. In metabonomics study, 35 depression-related metabolites were identified by 1H NMR and UHPLC-MS/MS metabonomics by comparing model and control groups. BP could significantly retrieve 17 depression-related metabolites. Thirteen depression-related metabolic pathways were found through Met-PA and BP could regulate seven metabolic pathways. Conclusions BP herb pair had significantly antidepressant effect, which provides a basis for further finding drug targets.
Databáze: OpenAIRE
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