Development of a disperse dye immunochromatographic test for the detection of antibodies against infectious bursal disease virus
| Autor: | Wei-Feng Chang, Yung-Chuan Liu, Kuang-Pin Hsiung, Min-Ying Wang, Shwu-Jinng Wang |
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| Rok vydání: | 2008 |
| Předmět: |
Immunology
Enzyme-Linked Immunosorbent Assay Antibodies Viral Infectious bursal disease virus Sensitivity and Specificity Infectious bursal disease Disperse dye Absorbance chemistry.chemical_compound medicine Animals Coloring Agents Poultry Diseases Reagent Strips Chromatography General Veterinary medicine.diagnostic_test biology Chromogenic medicine.disease Birnaviridae Infections Molecular biology Titer chemistry Immunoassay biology.protein Antibody Dyeing Chickens |
| Zdroj: | Veterinary immunology and immunopathology. 125(3-4) |
| ISSN: | 0165-2427 |
| Popis: | For investigating the feasibility of using disperse dyes as an immunoassay chromogenic marker, a disperse dye, DADISPERSE NAVY BLUE SP, was selected in analyzing antibody against infectious bursal disease virus (anti-IBDV). With the color intensity revealed in the disperse dye immunochromatographic test (DICT) strip as the objective function, the optimal dyeing conditions were found as follows: dye concentration absorbance (at λ max = 587 nm) = 3, pH 7, 50 °C, for 10 min. Under these conditions, the resultant dyed-antibody (rabbit anti-chicken) can produce an optimal color intensity reading of 55,054 on the strip. For performing qualitative immunoassay, chicken sera samples taken from different farms were used for the anti-IBDV titre assessment. The results of DICT strips showed very high sensitivity and specificity as compared to that analyzed by FlockChek enzyme linked immunosorbent assay (F-ELISA) kits. For quantitative immunoassay, it was found that the color intensity measured with DICT was linearly correlated to that of F-ELISA titre ( r 2 = 0.9687). Therefore, DICT was further applied to the detection of chicken anti-IBDV sera under vaccination in the farms. The average titres of the sampling groups exhibited a strong agreement to that of F-ELISA. Accordingly, the DICT method developed in this study, shown to be reliable, cheap and simple in both qualitative and quantitative immunoassays, is particularly suitable for point-of-need testing (PONT) in agricultural applications. |
| Databáze: | OpenAIRE |
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