d-Aspartate modulates melatonin synthesis in rat pinealocytes
| Autor: | Shougo Ishio, Mitsuko Hayashi, Yoshinori Moriyama, Shouki Yatsushiro, Takato Noumi, Akihito Yamaguchi, Hiroshi Yamada |
|---|---|
| Rok vydání: | 1998 |
| Předmět: |
endocrine system
medicine.medical_specialty Time Factors endocrine system diseases Arylamine N-Acetyltransferase Glutamic Acid Biology Pineal Gland Pinealocyte Melatonin chemistry.chemical_compound Pineal gland Organ Culture Techniques Biosynthesis Internal medicine Cyclic AMP medicine Animals Rats Wistar Neurotransmitter Incubation Cells Cultured Chromatography High Pressure Liquid Aspartic Acid Dose-Response Relationship Drug General Neuroscience nutritional and metabolic diseases Rats Endocrinology medicine.anatomical_structure Bucladesine chemistry Culture Media Conditioned Serotonin hormones hormone substitutes and hormone antagonists medicine.drug Endocrine gland |
| Zdroj: | Neuroscience Letters. 249:143-146 |
| ISSN: | 0304-3940 |
| DOI: | 10.1016/s0304-3940(98)00414-5 |
| Popis: | It has been known that pinealocytes contain the highest level of D-aspartate among various neuroendocrine cells in the rat. Here, we report that exogenous D-aspartate strongly inhibited norepinephrine-dependent melatonin synthesis in the rat pineal gland, the concentration required for 50% inhibition being 75 microM. This inhibition was due at least partly to decreased norepinephrine-dependent serotonin N-acetyltransferase activity. Upon incubation, D-aspartate was gradually released from pinealocytes and accumulated in the incubation medium as determined by high-performance liquid chromatography on a Pirkle-type chiral column. These results suggest that D-aspartate acts as a negative regulator for melatonin synthesis in the pineal gland. |
| Databáze: | OpenAIRE |
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