New GnRH-like peptide construct to optimize efficient immunocastration of male pigs by immunoneutralization of GnRH
Autor: | W.M.M. Schaaper, J.H.F. Erkens, M.H. Schuitemaker-de Weerd, Rob H. Meloen, A. van Nes, H.B. Oonk, J.A. Turkstra, J. H. M. Verheijden |
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Jazyk: | angličtina |
Rok vydání: | 1998 |
Předmět: |
Male
endocrine system medicine.medical_specialty Boar taint Ovalbumin Swine medicine.medical_treatment Polysorbates Gonadotropin-releasing hormone Biology Pharmacology Antigen-Antibody Reactions Gonadotropin-Releasing Hormone chemistry.chemical_compound Adjuvants Immunologic Internal medicine Testis medicine Animals Mineral Oil Instituut voor Dierhouderij en Diergezondheid Immunocontraception General Veterinary General Immunology and Microbiology ID-Lelystad Public Health Environmental and Occupational Health Androstenone Organ Size Hydrocarbons Vaccination ID Lelystad Anti-GnRH vaccine Infectious Diseases Castration Endocrinology chemistry ID-Lelystad Instituut voor Dierhouderij en Diergezondheid ID Lelystad Institute for Animal Science and Health Immunocastration Peptide vaccine Feasibility Studies Molecular Medicine Peptides Synthetic peptide vaccine Adjuvant Institute for Animal Science and Health |
Zdroj: | Vaccine 16 (1998) Vaccine, 16, 1074-1082 |
ISSN: | 0264-410X |
DOI: | 10.1016/s0264-410x(98)80101-1 |
Popis: | Castration of male pigs is routinely performed in order to prevent the occurrence of boar taint in pig carcasses. However, boar taint can also be eliminated by immunological castration using a synthetic peptide vaccine against GnRH. For pig farming, to make immunocastration a feasible alternative method to surgical castration, the composition of the vaccine has to be not only reliable and effective but also cost-efficient and safe. Previously the authors have developed an effective immunocastration vaccine by replacing the monomer GnRH by a much more immunogenic tandem peptide. However, this tandem-GnRH vaccine preparation needs Complete Freund's adjuvant and to be applied at a relatively high dose. Therefore, alternative antigens were designed to cope with this problem and tested with different adjuvants and dosages. An effective new antigen was designed based on a GnRH-tandem peptide, which was dimerized and modified in one amino acid position of the decapeptide to allow conjugation of this tandem-dimer to ovalbumin. In mild adjuvants and in low dosage, this antigen was very effective in reducing testis weight, serum LH and androstenone level in backfat. Thus, an improved immunocastration vaccine has been designed that is relatively cost-efficient and highly efficacious in two vaccinations at low dose. |
Databáze: | OpenAIRE |
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