New GnRH-like peptide construct to optimize efficient immunocastration of male pigs by immunoneutralization of GnRH

Autor: W.M.M. Schaaper, J.H.F. Erkens, M.H. Schuitemaker-de Weerd, Rob H. Meloen, A. van Nes, H.B. Oonk, J.A. Turkstra, J. H. M. Verheijden
Jazyk: angličtina
Rok vydání: 1998
Předmět:
Male
endocrine system
medicine.medical_specialty
Boar taint
Ovalbumin
Swine
medicine.medical_treatment
Polysorbates
Gonadotropin-releasing hormone
Biology
Pharmacology
Antigen-Antibody Reactions
Gonadotropin-Releasing Hormone
chemistry.chemical_compound
Adjuvants
Immunologic

Internal medicine
Testis
medicine
Animals
Mineral Oil
Instituut voor Dierhouderij en Diergezondheid
Immunocontraception
General Veterinary
General Immunology and Microbiology
ID-Lelystad
Public Health
Environmental and Occupational Health

Androstenone
Organ Size
Hydrocarbons
Vaccination
ID Lelystad
Anti-GnRH vaccine
Infectious Diseases
Castration
Endocrinology
chemistry
ID-Lelystad
Instituut voor Dierhouderij en Diergezondheid

ID Lelystad
Institute for Animal Science and Health

Immunocastration
Peptide vaccine
Feasibility Studies
Molecular Medicine
Peptides
Synthetic peptide vaccine
Adjuvant
Institute for Animal Science and Health
Zdroj: Vaccine 16 (1998)
Vaccine, 16, 1074-1082
ISSN: 0264-410X
DOI: 10.1016/s0264-410x(98)80101-1
Popis: Castration of male pigs is routinely performed in order to prevent the occurrence of boar taint in pig carcasses. However, boar taint can also be eliminated by immunological castration using a synthetic peptide vaccine against GnRH. For pig farming, to make immunocastration a feasible alternative method to surgical castration, the composition of the vaccine has to be not only reliable and effective but also cost-efficient and safe. Previously the authors have developed an effective immunocastration vaccine by replacing the monomer GnRH by a much more immunogenic tandem peptide. However, this tandem-GnRH vaccine preparation needs Complete Freund's adjuvant and to be applied at a relatively high dose. Therefore, alternative antigens were designed to cope with this problem and tested with different adjuvants and dosages. An effective new antigen was designed based on a GnRH-tandem peptide, which was dimerized and modified in one amino acid position of the decapeptide to allow conjugation of this tandem-dimer to ovalbumin. In mild adjuvants and in low dosage, this antigen was very effective in reducing testis weight, serum LH and androstenone level in backfat. Thus, an improved immunocastration vaccine has been designed that is relatively cost-efficient and highly efficacious in two vaccinations at low dose.
Databáze: OpenAIRE