A transcriptome-based genetic map of Chinese chestnut (Castanea mollissima) and identification of regions of segmental homology with peach (Prunus persica)
| Autor: | Sandra L. Anagnostakis, Thomas L. Kubisiak, Frederick V. Hebard, Margaret Staton, Bode A. Olukolu, Albert G. Abbott, Chris Smith, Guang Chen Fang, Nicholas Wheeler, Ronald R. Sederoff, Tatyana Zhebentyayeva, P. H. Sisco, C. D. Nelson |
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| Rok vydání: | 2012 |
| Předmět: |
0106 biological sciences
Population Castanea dentata Horticulture Biology Quantitative trait locus Plant disease resistance 01 natural sciences 03 medical and health sciences food Chestnut blight Botany Genetics Cryphonectria education Molecular Biology 030304 developmental biology Synteny 0303 health sciences education.field_of_study Forestry 15. Life on land biology.organism_classification food.food Genetic marker 010606 plant biology & botany |
| Zdroj: | Tree Genetics & Genomes. 9:557-571 |
| ISSN: | 1614-2950 1614-2942 |
| DOI: | 10.1007/s11295-012-0579-3 |
| Popis: | The Chinese chestnut (Castanea mollissima) carries resistance to Cryphonectria parasitica, the fungal pathogen inciting chestnut blight. The pathogen, introduced from Asia, devastated the American chestnut (Castanea dentata) through- out its native range early in the twentieth century. A highly informative genetic map of Chinese chestnut was constructed to extend genomic studies in the Fagaceae and to aid the intro- gression of Chinese chestnut blight resistance genes into Amer- ican chestnut. Two mapping populations were established with three Chinese chestnut parents, 'Mahogany', 'Nanking' ,a nd 'Vanuxem', totaling 337 progeny. The transcriptome-based ge- netic map was created with 329 simple sequence repeat and 1,064 single nucleotide polymorphism markers all derived from expressed sequence tag sequences. Genetic maps for each parent were developed and combined to establish 12 consensus linkage groups spanning 742 cM, providing the the most com- prehensive genetic map for a Fagaceae species to date. Over 75 % of the mapped markers from the Chinese chestnut con- sensus genetic map were placed on the physical map using overgo hybridization, providing a fully integrated genetic and physical map resource for Castanea spp. About half (57 %) of the Chinese chestnut genetic map could be assigned to regions of segmental homology with 58 % of the peach (Prunus per- sica) genome assembly. A three quantitative trait loci (QTL) model for blight resistance was verified using the new genetic markers and an existing interspecies (C. mollissima × C. den- tata)F2mapping population. Two of the blight resistance QTLs in chestnut shared synteny with two QTLs for powdery mildew resistance in peach, indicating the potential conservation of disease resistance genes at these loci. |
| Databáze: | OpenAIRE |
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