Melatonin improves methanol intoxication-induced oxidative liver injury in rats
| Autor: | Elif Oguz, Zehra Kurcer, Ersin Fadillioglu, Ercument Olmez, Mustafa Iraz, Mete Koksal, Fusun Baba |
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| Rok vydání: | 2007 |
| Předmět: |
Male
medicine.medical_specialty Protein oxidation Nitric oxide Melatonin Lipid peroxidation chemistry.chemical_compound Endocrinology Internal medicine medicine Animals Rats Wistar Liver injury chemistry.chemical_classification biology Methanol Glutathione peroxidase Malondialdehyde medicine.disease Rats Oxidative Stress Liver chemistry Myeloperoxidase biology.protein medicine.drug |
| Zdroj: | Journal of Pineal Research. 43:42-49 |
| ISSN: | 1600-079X 0742-3098 |
| Popis: | This study was performed to evaluate the effect of melatonin on methanol-induced liver injury. We evaluated the levels of malondialdehyde (MDA), protein carbonylation (PC), myeloperoxidase (MPO) activities and to assess lipid peroxidation, protein oxidation, neutrophil accumulation and nitrite which is a stable end product of nitric oxide respectively. We also studied superoxide dismutase, catalase, and glutathione peroxidase activities of liver tissue to evaluate the changes in the antioxidant status. Histopathological alterations were also determined. The experiment was performed on Wistar rats, which received intragastric 3 g/kg methanol as a 50% solution in isotonic saline once. After 6 and 24 hr all the drug received and intoxicated rats were killed under anesthesia. Pretreatment with melatonin (10 mg/kg) decreased the MDA levels significantly, restored the PC levels to the control, prevented the increase of nitrite level and MPO activity significantly and reversed to the control levels, prevented the reduction in all of the antioxidant enzyme activities. Additionally in melatonin treated group piecemeal necrosis, lobular lytic necrosis, and portal inflammation returned to normal histologic appearances when compared with methanol administration. In conclusion, melatonin has protective effects against methanol-induced hepatic injury. |
| Databáze: | OpenAIRE |
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