Molecular identification and genetic analysis of Rhipicephalus sanguineus sensu lato of dogs in Nigeria, West Africa

Autor: Joshua Kamani
Rok vydání: 2021
Předmět:
Zdroj: Experimental and Applied Acarology. 85:277-289
ISSN: 1572-9702
0168-8162
DOI: 10.1007/s10493-021-00664-w
Popis: The brown dog tick, Rhipicephalus sanguineus sensu lato, is a ubiquitous and taxonomically controversial pest of dogs with immense veterinary and public health significance. Genetic analyses of specimens from various geographical origins reveal intraspecific diversity within the taxon. Little information is available on the genetic characteristics of R. sanguineus s.l. in Nigeria, West Africa. In this study, 460 bp of the mitochondrial 16S rDNA gene of R. sanguineus s.l. collected from dogs in different ecological zones of Nigeria was amplified, sequenced and characterized. Phylogenetic and pairwise analyses were used to compare the sequences generated in this study to each other and to sequences in GenBank. The sequences in this study were highly similar (>98%) to each other and clustered with sequences of the R. sanguineus s.l. tropical lineage in GenBank. None of the sequences in this study clustered with the ‘southeastern Europe’ or temperate lineage. The mean intraspecific divergence among R. sanguineus s.l. in this study was 1.7% (range: 0−8.0%). Furthermore, the sequences in this study showed mean divergence of 1.5% (0−10%), 5.0% (3.8−13.9%) and 9.7% (6.9−19.8%) from sequences of the tropical, southeastern Europe and temperate lineages, respectively. Interestingly, sequences in this study showed a mean divergence of 9.3% (1.0−17.8%) from the Rhipicephalus sp. morphotype 4 (GenBank acc. nr. KC243850) earlier identified from cattle in Nigeria, suggesting diversity in this taxon in Nigeria. Further studies are needed to elucidate the veterinary and public health significance of R. sanguineus s.l. in Nigeria taking into cognizance the existence of intraspecific variation in vector competence.
Databáze: OpenAIRE
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