Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases
| Autor: | Dilokpimol, Adiphol, Verkerk, Bart, Li, Xinxin, Bellemare, Annie, Lavallee, Mathieu, Frommhagen, Matthias, Underlin, Emilie Nørmølle, Kabel, Mirjam A., Powlowski, Justin, Tsang, Adrian, de Vries, Ronald P., Translational Plant Biology, Sub Translational Plant Biology |
|---|---|
| Přispěvatelé: | Westerdijk Fungal Biodiversity Institute, Westerdijk Fungal Biodiversity Institute - Fungal Physiology, Translational Plant Biology, Sub Translational Plant Biology |
| Rok vydání: | 2022 |
| Předmět: |
Acetyl xylan esterase
Acetylesterase/chemistry acetyl xylan esterase hydroxycinnamic acid Biophysics Carboxylic Ester Hydrolases/chemistry Biochemistry Substrate Specificity carbohydrate esterase family 1 Structural Biology Levensmiddelenchemie Genetics feruloyl esterase Molecular Biology plant biomass VLAG Carbohydrate Esterase family 1 Plant biomass Food Chemistry Hydroxycinnamic acid Esterases Fungi food and beverages Cell Biology Esterases/genetics Feruloyl esterase Acetylesterase Xylans fungi Carboxylic Ester Hydrolases Xylans/metabolism |
| Zdroj: | Dilokpimol, A, Verkerk, B, Li, X, Bellemare, A, Lavallee, M, Frommhagen, M, Underlin, E N, Kabel, M A, Powlowski, J, Tsang, A & de Vries, R P 2022, ' Screening of novel fungal Carbohydrate Esterase family 1 enzymes identifies three novel dual feruloyl/acetyl xylan esterases ', FEBS Letters, vol. 596, no. 15, pp. 1932-1943 . https://doi.org/10.1002/1873-3468.14322 FEBS Letters, 596(15), 1932-1943. Elsevier B.V. FEBS Letters, 596(15), 1932-1943 FEBS Letters, 596(15), 1932. Wiley-Blackwell FEBS Letters 596 (2022) 15 |
| ISSN: | 1873-3468 0014-5793 |
| DOI: | 10.1002/1873-3468.14322 |
| Popis: | Feruloyl esterases (FAEs) and acetyl xylan esterases (AXEs) are important enzymes for plant biomass degradation and are both present in Carbohydrate Esterase family 1 (CE1) of the Carbohydrate-Active enZymes database. In this study, ten novel fungal CE1 enzymes from different subfamilies were heterologously produced and screened for their activity towards model and complex plant biomass substrates. CE1_1 enzymes possess AXE activity, while CE1_5 enzymes showed FAE activity. Two enzymes from CE1_2 and one from CE1_5 possess dual feruloyl/acetyl xylan esterase (FXE) activity, showing expansion of substrate specificity. The new FXEs from CE1 can efficiently release both feruloyl and acetyl residues from feruloylated xylan, making them particularly interesting novel components of industrial enzyme cocktails for plant biomass degradation. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Plný text