Rapid Cancer Diagnosis and Early Prognosis of Metastatic Risk Based on Mechanical Invasiveness of Sampled Cells
| Autor: | Y Horesh, Daphne Weihs, O Ben-Ishay, Yulia Merkher, G Shleifer, Z Abramov, Y Kluger |
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| Rok vydání: | 2020 |
| Předmět: |
Oncology
medicine.medical_specialty Cell Survival 0206 medical engineering Acrylic Resins Biomedical Engineering Breast Neoplasms 02 engineering and technology Disease Metastasis Cell Movement In vivo Pancreatic cancer Internal medicine Biopsy Tumor Cells Cultured medicine Humans Neoplasm Invasiveness Early Detection of Cancer Genetic testing medicine.diagnostic_test business.industry Cancer Prognosis medicine.disease 020601 biomedical engineering Pancreatic Neoplasms Histopathology business Gels |
| Zdroj: | Annals of Biomedical Engineering. 48:2846-2858 |
| ISSN: | 1573-9686 0090-6964 |
| DOI: | 10.1007/s10439-020-02547-4 |
| Popis: | We provide an innovative, bioengineering, mechanobiology-based approach to rapidly (2-h) establish the in vivo metastatic likelihood of patient tumor-samples, where results are in direct agreement with clinical histopathology and patient outcomes. Cancer-related mortality is mostly due to local recurrence or to metastatic disease, thus early prediction of tumor-cell-fate may critically affect treatment protocols and survival rates. Metastasis and recurrence risks are currently predicted by lymph-node status, tumor size, histopathology and genetic testing, however, these are not infallible and results may require days/weeks. We have previously observed that subpopulations of invasive cancer-cells will rapidly (1-2 h) push into the surface of physiological-stiffness, synthetic polyacrylamide gels, reaching to cell-scale depths, while normal or noninvasive cells do not considerably indent gels. Here, we evaluate the mechanical invasiveness of established breast and pancreatic cell lines and of tumor-cells from fresh, suspected pancreatic cancer tumors. The mechanical invasiveness matches the in vitro metastatic potential in cell lines as determined with Boyden chamber assays. Moreover, the mechanical invasiveness directly agrees with the clinical histopathology in primary-site, pancreatic-tumors. Thus, the rapid, patient-specific, early prediction of metastatic likelihood, on the time-scale of initial resection/biopsy, can directly affect disease management and treatment protocols. |
| Databáze: | OpenAIRE |
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