Cytotoxic effect of xyloglucan and oxovanadium (IV/V) xyloglucan complex in HepG2 cells

Autor: Carmen Lúcia de Oliveira Petkowicz, Leonardo Augusto dos Santos Escaliante, Silvia Maria Suter Correia Cadena, Guilhermina Rodrigues Noleto, Bianca Busato
Rok vydání: 2021
Předmět:
Carcinoma
Hepatocellular
Cell Survival
Chemical structure
Respiratory chain
Antineoplastic Agents
02 engineering and technology
Oxidative phosphorylation
Polysaccharide
Biochemistry
Oxidative Phosphorylation
03 medical and health sciences
chemistry.chemical_compound
Structural Biology
Multienzyme Complexes
Humans
Glycolysis
NADH
NADPH Oxidoreductases
Viability assay
Molecular Biology
Glucans
030304 developmental biology
Cell Proliferation
chemistry.chemical_classification
Membrane Potential
Mitochondrial
0303 health sciences
biology
L-Lactate Dehydrogenase
Plant Extracts
Liver Neoplasms
Fabaceae
General Medicine
Hep G2 Cells
021001 nanoscience & nanotechnology
biology.organism_classification
Molecular biology
Copaifera langsdorffii
Xyloglucan
Gene Expression Regulation
Neoplastic
chemistry
Xylans
Vanadates
0210 nano-technology
Oxidoreductases
Zdroj: International journal of biological macromolecules. 185
ISSN: 1879-0003
Popis: It is well known that the chemical structure of polysaccharides is important to their final biological effect. In this study we investigated the cytotoxic effect of xyloglucan from Copaifera langsdorffii seeds (XGC) and its complex with oxovanadium (XGC:VO) on hepatocellular carcinoma cells (HepG2). After 72 h of incubation, XGC and XGC:VO (200 μg/mL) reduced cell viability in ~20% and ~40%, respectively. At same conditions, only XGC:VO increased in ~20% the LDH enzyme release. In permeabilized cells, incubated with XGC and XGC:VO (200 μg/mL) for 72 h, NADH oxidase activity was reduced by ~45% with XGC and XGC:VO. The succinate oxidase activity was reduced by ~35% with XGC and ~65% with XGC:VO, evidencing that polysaccharide complexation with vanadium could intensify its effects on the respiratory chain. According to this result, the mitochondrial membrane potential was also reduced by ~9% for XGC and ~30% for XGC:VO, when compared to the control group. Interestingly, ATP levels were more elevated for XGC:VO in respect to XGC, probably due the enhance in glycolytic flux evidenced by increased levels of lactate. These results show that the xyloglucan complexation with oxovanadium (IV/V) potentiates the cytotoxic effect of the native polysaccharide, possibly by impairment of oxidative phosphorylation.
Databáze: OpenAIRE
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