Molecular Identification of Anoplophora glabripennis (Coleoptera: Cerambycidae) From Frass by Loop-Mediated Isothermal Amplification
| Autor: | Fabrizio Pennacchio, Tommaso Bruscoli, Elisabetta Rossi, Giovanni Cappellini, Domenico Rizzo, Chiara Salemi, Andrea Taddei, Linda Bartolini, Nicola Luchi, Daniele Da Lio |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
0301 basic medicine Asian longhorned beetle quarantine pest LAMP frass xylophagous insect Loop-mediated isothermal amplification Zoology frass 01 natural sciences 03 medical and health sciences LAMP Animals Phytosanitary certification Larva xylophagous insect Ecology biology Host (biology) Frass quarantine pest General Medicine biology.organism_classification Coleoptera Europe 010602 entomology 030104 developmental biology Molecular Diagnostic Techniques Asian longhorned beetle Insect Science North America Anoplophora PEST analysis Nucleic Acid Amplification Techniques Longhorn beetle |
| Zdroj: | Journal of economic entomology (Online) (2020). doi:10.1093/jee/toaa206 info:cnr-pdr/source/autori:Rizzo D., Taddei A., Da Lio D., Bruscoli T., Cappellini G., Bartolini L., Salemi C., Luchi N., Pennacchio F., Rossi E./titolo:Molecular Identification of Anoplophora glabripennis (Coleoptera: Cerambycidae) From Frass by Loop-Mediated Isothermal Amplification/doi:10.1093%2Fjee%2Ftoaa206/rivista:Journal of economic entomology (Online)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume |
| DOI: | 10.1093/jee/toaa206 |
| Popis: | Anoplophora glabripennis (Motschulsky, 1853), native to eastern Asia, is a destructive woodborer of many ornamental species, leading to the decline and the death of the attacked trees. In outbreak areas as Europe or North America, this pest is usually identified using morphological or molecular analyses of adult or larval specimens. However, the procedures for collecting A. glabripennis specimens from infested plants are too expensive and time consuming for routine screening. A noninvasive diagnostic tool based on frass discrimination is therefore crucial for the rapid identification of A. glabripennis at different development stages in the host. This article describes a rapid diagnostic protocol based on loop-mediated isothermal amplification (LAMP). DNA extracted from A. glabripennis frass was amplified with both visual and real-time LAMP and compared with those of nontarget species. The results show that the method is reliable and accurate and therefore could be a promising diagnostic tool in phytosanitary surveys. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|