Troglitazone attenuates hypoxia-induced injury in cultured term human trophoblasts
| Autor: | D. Michael Nelson, Rachel G. Humphrey, Yoel Sadovsky, Uriel Elchalal, Chaobin Hu, Steven D. Smith |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
medicine.medical_specialty
Time Factors medicine.drug_class Placenta Blotting Western Apoptosis Gestational Age Biology Sensitivity and Specificity Troglitazone Human placental lactogen Pregnancy Reference Values Risk Factors Internal medicine medicine Humans Chromans Thiazolidinedione Cells Cultured Obstetrics and Gynecology Trophoblast Hypoxia (medical) Immunohistochemistry Cell Hypoxia Trophoblasts Endocrinology medicine.anatomical_structure embryonic structures Female Thiazolidinediones Gonadotropin medicine.symptom medicine.drug |
| Zdroj: | American Journal of Obstetrics and Gynecology. 191:2154-2159 |
| ISSN: | 0002-9378 |
| DOI: | 10.1016/j.ajog.2004.05.009 |
| Popis: | The purpose of this study was to test the hypothesis that the thiazolidinedione troglitazone, a peroxisome proliferator activated receptor-gamma ligand, attenuates hypoxia-induced trophoblast injury.Cytotrophoblasts from 4 term human placentas were cultured in the presence or absence of 10 mumol/L troglitazone in either 20% oxygen (standard conditions) or 1% oxygen (hypoxic conditions) for variable periods before cell harvest. Medium beta-human chorionic gonadotropin and human placental lactogen were analyzed by enzyme-linked immunosorbent assay. Apoptosis was quantified by cytokeratin-18 cleavage products staining; p53 expression was examined by Western blot analysis.beta-human chorionic gonadotropin and human placental lactogen levels were/=2-fold higher in troglitazone-exposed cells at 16 hours of hypoxia, compared with vehicle control cells ( P.05). The apoptotic index was reduced by/=30% ( P.001), and the expression of p53 was 2-fold lower ( P.02) in troglitazone-exposed cells under hypoxia for/=16 hours but not different after24 hours of low oxygen.Troglitazone attenuates the influence of acute hypoxia on cultured term human trophoblasts. |
| Databáze: | OpenAIRE |
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