Global protein expression analysis of molecular markers of DS-1-47, a component of implantation-promoting traditional chinese medicine
| Autor: | Yan-ling Li, Yu Leng, Jing Li, Xiao-yan Zhang, Yunxia Wu, Yan-li Wu |
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| Rok vydání: | 2016 |
| Předmět: |
0301 basic medicine
Keratin 14 Proteome Biomedical Engineering Biology Proteomics Immunoglobulin light chain Biochemistry Biomaterials Mice 03 medical and health sciences Ovulation Induction Pregnancy Calcium-binding protein Heat shock protein Myosin Genetics Animals Embryo Implantation chemistry.chemical_classification Uterus 030104 developmental biology Enzyme chemistry Protein Expression Analysis Female Drugs Chinese Herbal |
| Zdroj: | Journal of Huazhong University of Science and Technology [Medical Sciences]. 36:910-915 |
| ISSN: | 1672-0733 |
| Popis: | This study investigated the molecular markers of DS-1-47, a component of an implantation- promoting traditional Chinese medicine consisting of Astragalus mongholicus, Atractylodes macrocephala, Scutellaria baicalensis and Dipsacales, in an attempt to clarify the molecular mechanism and action targets of DS-1-47. Controlled ovarian stimulation (COS) method was used to establish the implantation dysfunction models of mice. Animals were divided into normal pregnant group, COS model group and DS-1-47 group. Laser capture microdissection-double dimensional electrophoresis-mass spectrum (LCM-DE-MS) was used to analyze the uterine protein molecules that were possibly involved in the promotion of implantation. Twenty-three proteins in DS-1-47 group were significantly changed as compared to those in COS model group, with 7 proteins down-regulated and 16 proteins up-regulated. Except for some constituent proteins, the down-regulated proteins included collagen α-1 (VI) chain, keratin 7, keratin 14, myosin regulatory light chain 12B, myosin light polypeptide 9, heat shock protein β-7, and C-U-editing enzyme APOBEC-2; the up-regulated proteins included apolipoprotein A-I, calcium regulated protein-3, proliferating cell nuclear antigen, L-xylulose reductase, and calcium binding protein. These 23 proteins that were regulated by DS-1-47 represented a broad diversity of molecule functions. The down-regulated proteins were associated with stress and immune response, and those up-regulated proteins were related to proliferation. It was suggested that these proteins were important in regulating the uterine environment for the blastocyst implantation. By identification of DS-1-47 markers, proteomic analysis coupled with functional assays is demonstrated to be a promising approach to better understand the molecular mechanism of traditional Chinese medicine. |
| Databáze: | OpenAIRE |
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