Multidrug resistance increment in a human colon carcinoma cell line by colchicine
| Autor: | M. J. Ruiz Gómez, A. Souviron, L. Gil, M. Martínez Morillo |
|---|---|
| Rok vydání: | 2000 |
| Předmět: |
Vincristine
Physiology Mitomycin Antineoplastic Agents Drug resistance Adenocarcinoma Biology Pharmacology Vinblastine Biochemistry Flow cytometry chemistry.chemical_compound Tumor Cells Cultured medicine Humans Colchicine ATP Binding Cassette Transporter Subfamily B Member 1 P-glycoprotein medicine.diagnostic_test General Medicine Drug Resistance Multiple Multiple drug resistance Verapamil chemistry Drug Resistance Neoplasm Colonic Neoplasms biology.protein medicine.drug |
| Zdroj: | Journal of Physiology and Biochemistry. 56:33-38 |
| ISSN: | 1877-8755 1138-7548 |
| DOI: | 10.1007/bf03179774 |
| Popis: | The most important mechanism in drug resistance is the multidrug resistance (MDR) phenomenon. It is possible to select MDR cells by in vitro exposure to cytotoxic agents. The resistance is due to the hyperexpression of the P-glycoprotein (P-Gp) that take drugs out from the cells. In this study, a colchicine resistant subline (HCA-2/1cch) was selected from a human colon adenocarcinoma after a short period of drug exposure, as an in vitro model of drug resistance selection. These cells showed cross-resistance to other drugs, which were not present in the medium during selection. The relative resistance was 3.32 for colchicine, 3.15 for vinblastine, 2.62 for vincristine and 5.22 for mitomycin C. P-glycoprotein levels were assayed by flow cytometry. It was found that a significant increase of 2.35 and 1.59 had occurred in the peak and mean channel of fluorescence, respectively, indicating an increment of P-glycoprotein expression in relation to the parental line. Moreover, verapamil (10 microg/ml) produced a partial reversion of multidrug resistance. The sensitisation rates were 7.41 for colchicine, 1.25 for vinblastine, 2.36 for vincristine and 1.17 for mitomycin C. The data obtained suggest that colchicine exposure period (10 weeks) and dose (0.5 microg/ml) assayed were sufficient to produce an increment in multidrug resistance. This resistance could be due to higher level of P-Gp expression. |
| Databáze: | OpenAIRE |
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