Hepatic iron content corresponds with the susceptibility of lymphocytes to oxidative stress in neonatal pigs
| Autor: | Mikolaj A. Gralak, Rafał R. Starzyński, Teresa Bartłomiejczyk, Teresa Iwaneńko, Paweł Lipiński, Marcin Kruszewski, Jarosław Woliński, Romuald Zabielski |
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| Rok vydání: | 2008 |
| Předmět: |
Hypochlorous acid
DNA damage Swine Health Toxicology and Mutagenesis Lymphocyte Iron Oxidative phosphorylation Biology medicine.disease_cause Andrology chemistry.chemical_compound tert-Butylhydroperoxide Genetics medicine Animals Hepatic iron Lymphocytes Hydrogen peroxide X-Rays Hydrogen Peroxide Comet assay Oxidative Stress medicine.anatomical_structure chemistry Liver Immunology Leukocytes Mononuclear Comet Assay Oxidative stress DNA Damage |
| Zdroj: | Mutation research. 657(2) |
| ISSN: | 0027-5107 |
| Popis: | The pig is born with limited iron supplies. If not supplemented, piglets dramatically loose their body iron stores during the first few days of postnatal life. The aim of this study was to investigate the influence of hepatic iron content on susceptibility of blood cells to oxidative stress. Four 1-day-old and three 7-days-old animals were used in this study. The alkaline version of the comet assay was used to measure DNA damage. As expected, iron body stores of non-supplemented animals decrease significantly during the first 4 days of life. However, no difference in background DNA damage was found between untreated lymphocytes from these two groups of animals, despite the difference in their hepatic iron content. Interestingly, DNA damage induced by H2O2 and X-radiation in lymphocytes taken from 1-day-old piglets was significantly higher than in those taken from 7-days-old animals. In contrast, NaOCl or tert-butyl-hydroxide also induced significant amounts of DNA damage, but no differences between the two groups of piglets were found. Our data show that decreased hepatic iron content corresponds with decreased susceptibility of blood lymphocytes to oxidative stressors. |
| Databáze: | OpenAIRE |
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