Laser-printing of toner-based 96-microzone plates for immunoassays
| Autor: | Karoliny Almeida Oliveira, Wendell K. T. Coltro, Cristina Rodrigues de Oliveira, Lucimeire Antonelli da Silveira |
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| Rok vydání: | 2013 |
| Předmět: |
Detection limit
Materials science Laser printing biology Igm antibody Lasers Relative standard deviation Cell Culture Techniques Analytical chemistry Enzyme-Linked Immunosorbent Assay Repeatability Serum samples Biochemistry Analytical Chemistry Microplate Reader Mice Immunoglobulin M Electrochemistry biology.protein Animals Printing Environmental Chemistry Cells Cultured Spectroscopy |
| Zdroj: | The Analyst. 138:1114-1121 |
| ISSN: | 1364-5528 0003-2654 |
| DOI: | 10.1039/c2an36532d |
| Popis: | This work describes the quick and simple fabrication of toner-based 96-microzone plates by a direct-printing technology. The printer deposits a toner layer (ca. 5 μm thick) on the polyester surface which acts as a hydrophobic barrier to confine small volumes of sample on test zones (wells). A 96-microzone toner plate was explored to demonstrate its capability of performing enzyme-linked immunosorbent assay (ELISA). The detection of anti-immunoglobulin G (anti-IgG) and immunoglobulin M (IgM) antibodies has been successfully achieved in cell culture and serum samples, respectively. The use of a conventional microplate reader has allowed obtaining a limit of detection of 13 fmol of mouse IgG per zone on printed microplates. The IgM antibody has been detected in a serum sample collected from a patient infected with dengue virus. The detection of a primary infection has been provided by a microplate reader and also by a cell phone camera. Besides the bioanalytical feasibility, toner-based zones have shown good repeatability for inter-zone and intra-plate comparisons. The relative standard deviation (RSD) values for inter-zone (n = 12) and intra-plate (n = 3) comparisons were lower than 6% and 11%, respectively. Furthermore, it was found that the lifetime of each printed microplate depends on the storage temperature. The shelf life for devices stored at 10 °C has been estimated to be ca. four weeks. |
| Databáze: | OpenAIRE |
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