A high-throughput FTIR spectroscopy approach to assess adaptive variation in the chemical composition of pollen
| Autor: | Valeria Tafinstseva, Boris Zimmermann, Mikael Ohlson, Siri Fjellheim, Murat Bağcıoğlu, Achim Kohler |
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| Rok vydání: | 2017 |
| Předmět: |
Festuca
0106 biological sciences sparse partial least‐squares regression Anthoxanthum odoratum adaptation Poaceae medicine.disease_cause 010603 evolutionary biology 01 natural sciences Pollen Botany medicine Poa Chemical composition Ecology Evolution Behavior and Systematics Poa alpina Original Research Nature and Landscape Conservation flowering Ecology biology Anthoxanthum Fourier transform infrared spectroscopy food and beverages 15. Life on land biology.organism_classification ecology and environmental sciences grasses Agronomy Festuca ovina 010606 plant biology & botany |
| Zdroj: | Ecology and Evolution |
| ISSN: | 2045-7758 |
| Popis: | The two factors defining male reproductive success in plants are pollen quantity and quality, but our knowledge about the importance of pollen quality is limited due to methodological constraints. Pollen quality in terms of chemical composition may be either genetically fixed for high performance independent of environmental conditions, or it may be plastic to maximize reproductive output under different environmental conditions. In this study, we validated a new approach for studying the role of chemical composition of pollen in adaptation to local climate. The approach is based on high‐throughput Fourier infrared (FTIR) characterization and biochemical interpretation of pollen chemical composition in response to environmental conditions. The study covered three grass species, Poa alpina, Anthoxanthum odoratum, and Festuca ovina. For each species, plants were grown from seeds of three populations with wide geographic and climate variation. Each individual plant was divided into four genetically identical clones which were grown in different controlled environments (high and low levels of temperature and nutrients). In total, 389 samples were measured using a high‐throughput FTIR spectrometer. The biochemical fingerprints of pollen were species and population specific, and plastic in response to different environmental conditions. The response was most pronounced for temperature, influencing the levels of proteins, lipids, and carbohydrates in pollen of all species. Furthermore, there is considerable variation in plasticity of the chemical composition of pollen among species and populations. The use of high‐throughput FTIR spectroscopy provides fast, cheap, and simple assessment of the chemical composition of pollen. In combination with controlled‐condition growth experiments and multivariate analyses, FTIR spectroscopy opens up for studies of the adaptive role of pollen that until now has been difficult with available methodology. The approach can easily be extended to other species and environmental conditions and has the potential to significantly increase our understanding of plant male function. |
| Databáze: | OpenAIRE |
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