Silver nanowire exposure results in internalization and toxicity to Daphnia magna
| Autor: | Philipp Antczak, Robert B. Reed, Shaul Aloni, Francesco Falciani, Nadeeka Karunaratne, P. T. Luong, Daniel Thomas Nowinski, James F. Ranville, Chris D. Vulpe, Abderrahmane Tagmount, Alexandre Loguinov, Xin Xin Lin, Christopher P. Higgins, Leona D. Scanlan, Christine Tran, Benjamin Gilbert, Don Pham |
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| Rok vydání: | 2013 |
| Předmět: |
Materials science
Silver Silicon dioxide Daphnia magna General Physics and Astronomy Nanoparticle Metal Nanoparticles engineering.material Daphnia Article Nanomaterials Lethal Dose 50 chemistry.chemical_compound Coating Hemolymph Toxicity Tests Toxicity Tests Acute Animals General Materials Science Inductively coupled plasma mass spectrometry reproductive and urinary physiology biology Nanowires Gene Expression Profiling fungi General Engineering Povidone Silver Compounds biology.organism_classification Silicon Dioxide Acute toxicity Oxygen Chemical engineering chemistry Environmental chemistry engineering Water Pollutants Chemical Aluminum |
| Zdroj: | ACS nano. 7(12) |
| ISSN: | 1936-086X |
| Popis: | Nanowires (NWs), high-aspect-ratio nanomaterials, are increasingly used in technological materials and consumer products and may have toxicological characteristics distinct from nanoparticles. We carried out a comprehensive evaluation of the physico-chemical stability of four silver nanowires (AgNWs) of two sizes and coatings and their toxicity to Daphnia magna. Inorganic aluminum-doped silica coatings were less effective than organic poly(vinyl pyrrolidone) coatings at preventing silver oxidation or Ag+ release and underwent a significant morphological transformation within one-hour following addition to low ionic strength Daphnia growth media. All AgNWs were highly toxic to D. magna but less toxic than ionic silver. Toxicity varied as a function of AgNW dimension, coating and solution chemistry. Ag+ release in the media could not account for observed AgNW toxicity. Single-particle inductively coupled plasma mass spectrometry (spICPMS) distinguished and quantified dissolved and nanoparticulate silver in microliter-scale volumes of Daphnia magna hemolymph with a limit of detection of approximately 10 ppb. The silver levels within the hemolymph of Daphnia exposed to both Ag+ and AgNW met or exceeded the initial concentration in the growth medium, indicating effective accumulation during filter feeding. Silver-rich particles were the predominant form of silver in hemolymph following exposure to both AgNWs and Ag+. Scanning electron microscopy (SEM) imaging of dried hemolymph found both AgNWs and silver precipitates that were not present in the AgNW stock or the growth medium. Both organic and inorganic coatings on the AgNW were transformed during ingestion or absorption. Pathway, gene ontology and clustering analyses of gene expression response indicated effects of AgNWs distinct from ionic silver on Daphnia magna. |
| Databáze: | OpenAIRE |
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