Early occurrence of human cytomegalovirus infection after bone marrow transplantation as demonstrated by the polymerase chain reaction technique
| Autor: | Johannes G. Saal, Gerhard Ehninger, M Steidle, Hermann Einsele, Angelika Vallbracht, Claudia A. Müller |
|---|---|
| Rok vydání: | 1991 |
| Předmět: |
Human cytomegalovirus
Pathology medicine.medical_specialty biology Viral culture Immunology Congenital cytomegalovirus infection virus diseases Viremia Cell Biology Hematology medicine.disease biology.organism_classification Virology Biochemistry law.invention Transplantation medicine.anatomical_structure Betaherpesvirinae law medicine Bone marrow Polymerase chain reaction |
| Zdroj: | Blood. 77:1104-1110 |
| ISSN: | 1528-0020 0006-4971 |
| DOI: | 10.1182/blood.v77.5.1104.bloodjournal7751104 |
| Popis: | Twenty-eight patients undergoing bone marrow transplantation (BMT) were followed-up at weekly intervals from day -10 to discharge from hospital after BMT for human cytomegalovirus (HCMV) infection using polymerase chain reaction (PCR), slot-blot hybridization, and conventional virus culture. High specificity of the PCR assay applied could be shown by failure to amplify DNA extracted from a wide range of other viruses frequently infecting marrow transplant recipients. The PCR technique allowed us to diagnose viremia and viruria in 20 (83%) of 24 seropositive patients after BMT, whereas culture assays showed 16 (67%) of 24 of these patients to be viruric and 9 (37%) of 24 cases to be viremic. Slot-blot hybridization showed a frequency of viruria and viremia in 12 (50%) of 24 seropositive patients. By application of PCR techniques, HCMV detection could be achieved even in the very early posttransplant period. HCMV was detected in five patients even before the onset of clinical symptoms of acute graft-versus-host disease. Analysis by PCR techniques of 33 organ biopsy specimens from patients after BMT showed the presence of HCMV in 13 of 14 liver samples obtained from patients with HCMV viremia; three liver specimens from patients without viremia were negative by all the techniques applied. HCMV could also be demonstrated in postmortem lung biopsy specimens from all patients (n = 10) with interstitial pneumonia. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|