Genetic structure and insecticide resistance characteristics of fall armyworm populations invading China

Autor: Yu-Ying Jiang, Weipeng Quan, Kenneth Wilson, Chao Wu, Wanqiang Qian, Bo Liu, Swapan Chakrabarty, Gilson Chipabika, Sheng-yuan Zhao, W. Zheng, Dandan Zhang, Wei Fan, Guirong Wang, Bei Liu, Jie Liu, Xinyue Liang, Amy Withers, Christopher M. Jones, Minghui Jin, Judith A. Smith, Yutao Xiao, Xiaolin Liu, Emmanuelle d'Alençon, Kiwoong Nam, Zaiyuan Li, Xianming Yang, Donald L. Kachigamba, Lei Zhang, Pengjun Xu, Conghui Liu, Kongming Wu
Přispěvatelé: Chinese Academy of Agricultural Sciences (CAAS), Chinese Academy of Sciences [Beijing] (CAS), Lancaster University, Malawi Liverpool Wellcome Trust Clinical Research Programme (MLW), Liverpool School of Tropical Medicine (LSTM)-University of Liverpool-Wellcome Trust-University of Malawi, University of Central Lancashire [Preston] (UCLAN), Zambia Agriculture Research Institute, Partenaires INRAE, Bvumbwe Research Station, Diversité, Génomes & Interactions Microorganismes - Insectes [Montpellier] (DGIMI), Université de Montpellier (UM)-Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement (INRAE), Natl Agrotech Extens & Serv Ctr, Novogene Bioinformatics Institute, Grandomics Biosciences
Jazyk: angličtina
Rok vydání: 2020
Předmět:
0106 biological sciences
0301 basic medicine
resistance risk
China
Nuclear gene
Population
Genome
Insect
Spodoptera
010603 evolutionary biology
01 natural sciences
Genome
Zea mays
Insecticide Resistance
qx_560
03 medical and health sciences
Hemolysin Proteins
South Africa
Bacterial Proteins
Bacillus thuringiensis
qx_600
Genetics
Animals
[SDV.BBM]Life Sciences [q-bio]/Biochemistry
Molecular Biology
Resource Article
education
Permanent Genetic Resources
subpopulations
Ecology
Evolution
Behavior and Systematics
education.field_of_study
qx_4
Genetically modified maize
biology
F410
Strain (biology)
RESOURCE ARTICLES
population structure
Spodoptera frugiperda
biology.organism_classification
Plants
Genetically Modified
Endotoxins
030104 developmental biology
Genetic structure
Fall armyworm
qu_450
[SDE.BE]Environmental Sciences/Biodiversity and Ecology
gene insertion
Biotechnology
Zdroj: Molecular Ecology Resources
Molecular Ecology Resources, Wiley/Blackwell, 2020, ⟨10.1111/1755-0998.13219⟩
ISSN: 1755-0998
1755-098X
Popis: The rapid wide-scale spread of fall armyworm (Spodoptera frugiperda) has caused serious crop losses34 globally. However, differences in the genetic background of subpopulations and the mechanisms of rapid adaptation behind the invasion are still not well understood. Here we report the assembly of a 390.38-M chromosome-level genome of fall armyworm using Pacific Bioscience (PacBio) and Hi-C sequencing technologies with scaffold N50 of 12.7 M consisting of 22260 annotated protein-coding genes. Genome-wide resequencing of 103 samples from 16 provinces in China revealed that the fall armyworm population comprises a complex inter-strain hybrid, mainly with the corn-strain genetic background and less of the rice-strain, which highlights the inaccuracy of strain identification using mitochondrial or Triosephosphate isomerase (Tpi) genes. Analysis of genes related to pesticide- and Bt-resistance showed that the risk of fall armyworm developing resistance to conventional pesticides is very high. Laboratory bioassay results showed that insects invading China carry resistance to organophosphate and pyrethroid pesticides, but are sensitive to genetically modified maize expressing Bacillus thuringiensis (Bt) toxins Cry1Ab in field experiments. Additionally, two mitochondrial fragments are inserted into the nuclear genome, and the insertion event occurred after the differentiation of the two strains. This study represents a valuable advance toward improving management strategies for fall armyworm.
Databáze: OpenAIRE
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