Expression of SGLT-1 in preneoplastic and neoplastic lesions of the head and neck
| Autor: | Burkhard M, Helmke, Christoph, Reisser, Mario, Idzko, Gerhard, Dyckhoff, Christel, Herold-Mende, Mario, Idzkoe |
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| Rok vydání: | 2003 |
| Předmět: |
Cancer Research
Pathology medicine.medical_specialty Monosaccharide Transport Proteins Glucose uptake Cell Blotting Western Biology Sodium-Glucose Transporter 1 Cell Line Tumor Carcinoma medicine Humans RNA Messenger RNA Neoplasm Membrane Glycoproteins Reverse Transcriptase Polymerase Chain Reaction Glucose transporter medicine.disease Immunohistochemistry Staining Blot stomatognathic diseases medicine.anatomical_structure Oncology Epidermoid carcinoma Head and Neck Neoplasms Carcinoma Squamous Cell Oral Surgery HT29 Cells Precancerous Conditions |
| Zdroj: | Oral oncology. 40(1) |
| ISSN: | 1368-8375 |
| Popis: | Tumors show an increased glucose uptake that is mediated by glucose transport proteins. We have analyzed the expression of the sodium-dependent glucose co-transporters SGLT-1 and-2 in short-term cultures of squamous cell carcinomas of the head and neck (HNSCC) by RT-PCR. Distribution of the SGLT-1 protein in HNSCC tissues was investigated by immunohistochemistry. While we observed in 17/36 HNSCC short-term cultures the SGLT-1 mRNA, we found no SGLT-2 expression. SGLT-1 mRNA expression occurred preferentially in cultures originating from moderately and well differentiated HNSCC. In tumor tissues a heterogeneous SGLT-1 staining restricted to differentiated tumor cells was seen in 27/30 HNSCC analyzed. In normal mucosa SGLT-1 staining was also confined to differentiated compartments and lacked in dysplastic areas. Our data indicate a differentiation-dependent expression of SGLT-1 in HNSCC. This is important knowledge for the planning of glucose-targeting therapies and suggest SGLT-1 as a differentiation marker in head and neck tissues. |
| Databáze: | OpenAIRE |
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