Resveratrol encapsulation in high molecular weight chitosan-based nanogels for applications in ocular treatments: Impact on human ARPE-19 culture cells
| Autor: | Guadalupe Eugenia Garcia Liñares, Agustina Alaimo, Sofía L. Acebedo, Leonardo Lizarraga, Carla Cecilia Spagnuolo, Mariana Carolina Di Santo, Fernanda Elias, Karina Dafne Martínez, Mayra Alejandra Castañeda Cataña, Florencia Solana Buosi, Oscar E. Pérez |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Antioxidant
Biocompatibility medicine.medical_treatment media_common.quotation_subject Population Nanogels Retinal Pigment Epithelium 02 engineering and technology Resveratrol Biochemistry Antioxidants Cell Line Chitosan 03 medical and health sciences chemistry.chemical_compound Nanocapsules Structural Biology medicine Humans Internalization education Molecular Biology 030304 developmental biology media_common 0303 health sciences education.field_of_study General Medicine 021001 nanoscience & nanotechnology chemistry Drug delivery Biophysics Nanocarriers 0210 nano-technology |
| Zdroj: | International Journal of Biological Macromolecules. 165:804-821 |
| ISSN: | 0141-8130 |
| DOI: | 10.1016/j.ijbiomac.2020.09.234 |
| Popis: | Oxidative stress and inflammation play a pivotal role in ocular diseases. Resveratrol (RSV) is a natural bioactive that has recently attracted attention due to it potent antioxidant and anti-inflammatory properties. However, RSV presents poor aqueous solubility and chemical instability. Besides, effective drug delivery to the posterior segment of the eye is challenge. Nanotechnology emerges as a possible solution to improve both limitations. Here, we developed and characterized nanogels (NG) based on high molecular weight chitosan (HCS) crosslinked with sodium tripolyphosphate. The distribution size of NG presented a major population around 140 nm with a ζ-potential value of 32 ± 2 mV. TEM and AFM images showed that NG exhibited a rounded morphology. RSV encapsulation efficiency was 59 ± 1%. Photodegradation experiments showed that HCS by its own protects RSV from UV light-induced degradation. Biocompatibility assays revealed that NG were not cytotoxic neither inflammatory in human retinal pigment epithelial cells (ARPE-19), which constitutes the outer blood-retinal barrier. After cellular internalization, we report an endo-lysosomal escape of NG, which is crucial for efficient nanocarriers delivery systems. In conclusion, we envision that HCS based NG could constitute novel carriers for RSV, opening the possibility of its application in ocular diseases. |
| Databáze: | OpenAIRE |
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