Towards objective hand hygiene technique assessment: validation of the ultraviolet-dye-based hand-rubbing quality assessment procedure

Autor: Száva Bánsághi, László Szilágyi, Peter Szeremy, Tamas Haidegger, Ákos Lehotsky, György Wéber
Rok vydání: 2017
Předmět:
Zdroj: The Journal of hospital infection. 97(1)
ISSN: 1532-2939
Popis: Ultraviolet spectrum markers are widely used for hand hygiene quality assessment, although their microbiological validation has not been established. A microbiology-based assessment of the procedure was conducted. Twenty-five artificial hand models underwent initial full contamination, then disinfection with UV-dyed hand-rub solution, digital imaging under UV-light, microbiological sampling and cultivation, and digital imaging of the cultivated flora were performed. Paired images of each hand model were registered by a software tool, then the UV-marked regions were compared with the pathogen-free sites pixel by pixel. Statistical evaluation revealed that the method indicates correctly disinfected areas with 95.05% sensitivity and 98.01% specificity.
Databáze: OpenAIRE