Genomic instability in human lymphocytes from male users of crack cocaine
| Autor: | Felix Kessler, Thiago Aley Brites de Freitas, Flavio Pechansky, Gisele Gomes de Andrade, César Luis Reichert, Caroline Brunetto de Farias, Roberta Passos Palazzo, Fabiana Michelsen de Andrade, Sandra Leistner-Segal, Sharbel Weidner Maluf |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2014 |
| Předmět: |
Genome instability
Adult Male medicine.medical_specialty Usuários de drogas Adolescent DNA damage Nuclear Envelope Health Toxicology and Mutagenesis lcsh:Medicine Inflammation Instabilidade genômica Biology Article Genomic Instability Drug withdrawal Cocaine-Related Disorders comet assay Internal medicine mental disorders medicine Cocaína crack Humans micronucleus Linfócitos Lymphocytes Masculino Comet assay Micronuclei Chromosome-Defective Genetics Cell Nucleus crack cocaine Crack cocaine Micronucleus Tests lcsh:R Public Health Environmental and Occupational Health Cancer Middle Aged medicine.disease Endocrinology Micronucleus drug withdrawal Micronucleus test medicine.symptom Brazil |
| Zdroj: | Repositório Institucional da UFRGS Universidade Federal do Rio Grande do Sul (UFRGS) instacron:UFRGS International Journal of Environmental Research and Public Health, Vol 11, Iss 10, Pp 10003-10015 (2014) International Journal of Environmental Research and Public Health Volume 11 Issue 10 Pages 10003-10015 |
| Popis: | Recent research suggests that crack cocaine use alters systemic biochemical markers, like oxidative damage and inflammation markers, but very few studies have assessed the potential effects of crack cocaine at the cellular level. We assessed genome instability by means of the comet assay and the cytokinesis-block micronucleus technique in crack cocaine users at the time of admission to a rehabilitation clinic and at two times after the beginning of withdrawal. Thirty one active users of crack cocaine and forty control subjects were evaluated. Comparison between controls and crack cocaine users at the first analysis showed significant differences in the rates of DNA damage (p = 0.037). The frequency of micronuclei (MN) (p < 0.001) and nuclear buds (NBUDs) (p < 0.001) was increased, but not the frequency of nucleoplasmic bridges (NPBs) (p = 0.089). DNA damage decreased only after the end of treatment (p < 0.001). Micronuclei frequency did not decrease after treatment, and nuclear buds increased substantially. The results of this study reveal the genotoxic and mutagenic effects of crack cocaine use in human lymphocytes and pave the way for further research on cellular responses and the possible consequences of DNA damage, such as induction of irreversible neurological disease and cancer. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|