Engineering of Self-Assembled Fibronectin Matrix Protein and Its Effects on Mesenchymal Stem Cells
| Autor: | Le B. Hang Pham, Sujin Lee, Yie-Ri Yoo, Hae-Won Kim, Jun-Hyeog Jang, Ye-Rang Yun |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
Male
cellular activity Cellular differentiation Recombinant Fusion Proteins Protein Engineering Catalysis Article Inorganic Chemistry Focal adhesion Rats Sprague-Dawley peptide amphiphile lcsh:Chemistry fibronectin rat mesenchymal stem cells Peptide amphiphile Cell Adhesion Animals Physical and Theoretical Chemistry Cell adhesion Molecular Biology lcsh:QH301-705.5 Spectroscopy Cells Cultured Cell Proliferation biology Cell adhesion molecule Organic Chemistry Mesenchymal stem cell Cell Differentiation Mesenchymal Stem Cells General Medicine Adhesion Molecular biology Peptide Fragments Computer Science Applications Cell biology Fibronectins Rats Fibronectin lcsh:Biology (General) lcsh:QD1-999 cellularactivity biology.protein |
| Zdroj: | International Journal of Molecular Sciences, Vol 16, Iss 8, Pp 19645-19656 (2015) International Journal of Molecular Sciences Volume 16 Issue 8 Pages 19645-19656 INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES(16): 8 |
| ISSN: | 1422-0067 |
| Popis: | Fibronectin (FN) contributes to cell adhesion, proliferation, and differentiation in various cell types. To enhance the activity of fibronectin at the sites of focal adhesion, we engineered a novel recombinant fibronectin (FNIII10) fragment connected to the peptide amphiphile sequence (PA), LLLLLLCCCGGDS. In this study, the effects of FNIII10-PA on rat mesenchymal stem cells (rMSCs) were compared with those of FNIII10. FNIII10-PA showed the prominent protein adhesion activity. In addition, FNIII10-PA showed a significantly higher effect on adhesion, proliferation, and differentiation of rMSCs than FNIII10. Taken together, the FNIII10-containing self-assembled sequence enhanced rMSCs adhesion, proliferation, and differentiation. |
| Databáze: | OpenAIRE |
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