FHOD-1 is the only formin in Caenorhabditis elegans that promotes striated muscle growth and Z-line organization in a cell autonomous manner
| Autor: | David Pruyne, Arianna Laszlo, Sumana Sundaramurthy, SarahBeth Votra, Tim Davies |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2020 |
| Předmět: |
Fetal Proteins
0303 health sciences biology Mutant Formins Cell Biology biology.organism_classification Embryonic stem cell Sarcomere Article Muscle Striated Muscle hypertrophy Cell biology Sarcomerogenesis 03 medical and health sciences 0302 clinical medicine Structural Biology biology.protein Myocyte Animals Caenorhabditis elegans 030217 neurology & neurosurgery 030304 developmental biology |
| Zdroj: | Cytoskeleton (Hoboken) Cytoskeleton, 2020, Vol.77(10), pp.422-441 [Peer Reviewed Journal] |
| Popis: | The striated body wall muscles of Caenorhabditis elegans are a simple model for sarcomere assembly. Previously, we observed deletion mutants for two formin genes, fhod‐1 and cyk‐1, develop thin muscles with abnormal dense bodies (the sarcomere Z‐line analogs). However, this work left in question whether these formins work in a muscle cell autonomous manner, particularly since cyk‐1(Δ) deletion has pleiotropic effects on development. Using a fast acting temperature‐sensitive cyk‐1(ts) mutant, we show here that neither post‐embryonic loss nor acute loss of CYK‐1 during embryonic sarcomerogenesis cause lasting muscle defects. Furthermore, mosaic expression of CYK‐1 in cyk‐1(Δ) mutants is unable to rescue muscle defects in a cell autonomous manner, suggesting muscle phenotypes caused by cyk‐1(Δ) are likely indirect. Conversely, mosaic expression of FHOD‐1 in fhod‐1(Δ) mutants promotes muscle cell growth and proper dense body organization in a muscle cell autonomous manner. As we observe no effect of loss of any other formin on muscle development, we conclude FHOD‐1 is the only worm formin that directly promotes striated muscle development, and the effects on formin loss in C. elegans are surprisingly modest compared to other systems. |
| Databáze: | OpenAIRE |
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