Evaluation of phenotypic and functional stability of RAW 264.7 cell line through serial passages
| Autor: | Paulina Sawicka, Bartłomiej Taciak, Maciej Białasek, Łukasz Kiraga, Zuzanna Sas, Agata Braniewska, Magdalena Król, Tomasz P. Rygiel |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
Myeloid Cellular differentiation lcsh:Medicine Biology Nitric Oxide Real-Time Polymerase Chain Reaction Flow cytometry Mice 03 medical and health sciences Phagocytosis Functional stability medicine Animals lcsh:Science Gene Multidisciplinary medicine.diagnostic_test Macrophages Monocyte lcsh:R Proteins Phenotype Cell biology RAW 264.7 Cells 030104 developmental biology medicine.anatomical_structure Cell culture lcsh:Q |
| Zdroj: | PLoS ONE, Vol 13, Iss 6, p e0198943 (2018) |
| ISSN: | 1932-6203 |
| Popis: | Established cell lines are widely used in research, however an appealing question is the comparability of the cells between various laboratories, their characteristics and stability in time. Problematic is also the cell line misidentification, genetic and phenotypic shift or Mycoplasma contamination which are often forgotten in research papers. The monocyte/macrophage-like cell line RAW 264.7 has been one of the most commonly used myeloid cell line for more than 40 years. Despite its phenotypic and functional stability is often discussed in literature or at various scientific discussion panels, their stability during the consecutive passages has not been confirmed in any solid study. So far, only a few functional features of these cells have been studied, for example their ability to differentiate into osteoclasts. Therefore, in the present paper we have investigated the phenotype and functional stability of the RAW 264.7 cell line from passage no. 5 till passage no. 50. We found out that the phenotype (expression of particular macrophage-characteristic genes and surface markers) and functional characteristics (phagocytosis and NO production) of RAW 264.7 cell line remains stable through passages: from passage no. 10 up to passage no. 30. Overall, our results indicated that the RAW 264.7 cell line should not be used after the passage no. 30 otherwise it may influence the data reliability. |
| Databáze: | OpenAIRE |
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