Phenylalanine regulates initiation of digestive enzyme mRNA translation in pancreatic acinar cells and tissue segments in dairy calves
| Autor: | Long Guo, Chen Zheng, Shi Ming Liu, J.H. Yao, Chuan Jiang Cai, Hui Bin Tian, Y.C. Cao, Jing Shen |
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| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
phenylalanine Primary Cell Culture Biophysics P70-S6 Kinase 1 Acinar Cells translation regulation Biochemistry Gene Expression Regulation Enzymologic 03 medical and health sciences Translational regulation medicine Initiation factor Animals Trypsin pancreas Phosphorylation Molecular Biology Research Articles biology Chemistry Ribosomal Protein S6 Kinases mammalian target of rapamycin (mTOR) Eukaryotic initiation factor 4E binding Epithelial Cells Cell Biology dairy calves Lipase Molecular biology α-amylase 030104 developmental biology medicine.anatomical_structure Ribosomal protein s6 Protein Biosynthesis Digestive enzyme biology.protein Cattle alpha-Amylases Pancreas medicine.drug Research Article Signal Transduction |
| Zdroj: | Bioscience Reports |
| ISSN: | 1573-4935 0144-8463 |
| Popis: | As new nutritional strategies for ruminant are designed to change production efficiency by improving the supply of rumen protect protein, lipid, and even starch, the digestive system must fit to utilize these increased nutrient supplies, especially the pancreas. The objective of this study was to investigate the effects of phenylalanine (Phe) on digestive enzymes synthesis or secretion and cellular signaling in pancreatic acinar (PA) cells of dairy calves. The PA cells isolated from fresh pancreas of dairy calves, and cultured in completed RIPA 1640 medium with no fetal serum but 0, 0.15 and 0.45 mM Phe at 37°C in CO2 incubator for 120 min. The pancreatic tissue segments (PTS) was cut approximately 2 × 2 mm from the fresh pancreas, and incubated in oxygenated Krebs-Ringer bicarbonate (KRB) buffer containing 0 or 0.35 mM Phe at 39°C for 180 min, and the samples were collected every 60 min after incubation. In PA cells, Phe increased (P < 0.05) the α-amylase secretion and mRNA expression, the phosphorylation of ribosomal protein S6 kinase 1 (S6K1) and eukaryotic initiation factor 4E binding protein 1 (4EBP1). In PTS, the Phe increased (P < 0.05) α-amylase and trypsin synthesis, secretion and mRNA expression, as well as the phosphorylation of S6K1 and 4EBP1. Conclusively, these results suggested that Phe regulates the synthesis or secretion of α-amylase, trypsin and lipase through mRNA translation initiation factors – S6K1 and 4EBP1. |
| Databáze: | OpenAIRE |
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