A major QTL corresponding to the Rk locus for resistance to root-knot nematodes in cowpea (Vigna unguiculata L. Walp.)
| Autor: | Arsenio Ndeve, Jansen R. P. Santos, W. C. Matthews, Bao-Lam Huynh, Philip A. Roberts, Jeffrey D. Ehlers, Timothy J. Close, Mitchell R. Lucas |
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| Jazyk: | angličtina |
| Rok vydání: | 2015 |
| Předmět: |
0106 biological sciences
0301 basic medicine Technology Positional cloning Genotype Genetic Linkage Population Plant Biology & Botany Quantitative Trait Loci Locus (genetics) Quantitative trait locus Plant disease resistance Crosses 01 natural sciences Plant Roots Polymorphism Single Nucleotide Vigna 03 medical and health sciences Genetic Genetics Animals Tylenchoidea Polymorphism education Crosses Genetic Disease Resistance Plant Diseases education.field_of_study biology Agricultural and Veterinary Sciences food and beverages Chromosome Mapping Fabaceae General Medicine R gene Single Nucleotide Biological Sciences biology.organism_classification 030104 developmental biology Phenotype Backcrossing Original Article Agronomy and Crop Science 010606 plant biology & botany Biotechnology |
| Zdroj: | TAG. Theoretical and Applied Genetics. Theoretische Und Angewandte Genetik TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik, vol 129, iss 1 |
| ISSN: | 1432-2242 0040-5752 |
| Popis: | Key message Genome resolution of a major QTL associated with the Rk locus in cowpea for resistance to root-knot nematodes has significance for plant breeding programs and R gene characterization. Abstract Cowpea (Vigna unguiculata L. Walp.) is a susceptible host of root-knot nematodes (Meloidogyne spp.) (RKN), major plant-parasitic pests in global agriculture. To date, breeding for host resistance in cowpea has relied on phenotypic selection which requires time-consuming and expensive controlled infection assays. To facilitate marker-based selection, we aimed to identify and map quantitative trait loci (QTL) conferring the resistance trait. One recombinant inbred line (RIL) and two F2:3 populations, each derived from a cross between a susceptible and a resistant parent, were genotyped with genome-wide single nucleotide polymorphism (SNP) markers. The populations were screened in the field for root-galling symptoms and/or under growth-chamber conditions for nematode reproduction levels using M. incognita and M. javanica biotypes. One major QTL was mapped consistently on linkage group VuLG11 of each population. By genotyping additional cowpea lines and near-isogenic lines derived from conventional backcrossing, we confirmed that the detected QTL co-localized with the genome region associated with the Rk locus for RKN resistance that has been used in conventional breeding for many decades. This chromosomal location defined with flanking markers will be a valuable target in marker-assisted breeding and for positional cloning of genes controlling RKN resistance. Electronic supplementary material The online version of this article (doi:10.1007/s00122-015-2611-0) contains supplementary material, which is available to authorized users. |
| Databáze: | OpenAIRE |
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