The human NTERA2 neural cell line generates neurons on growth under neural stem cell conditions and exhibits characteristics of radial glial cells
| Autor: | J De Weille, Loic P. Deleyrolle, Jean-Philippe Hugnot, F Sandillon, Monique Saunier, Cecile Dromard, Sophie Marchal-Victorion, Alain Privat |
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| Rok vydání: | 2003 |
| Předmět: |
Cellular differentiation
Cell Culture Techniques Fluorescent Antibody Technique Nerve Tissue Proteins Biology Models Biological Cell Line Cellular and Molecular Neuroscience Mice Neurosphere Spheroids Cellular Animals Humans Cell Lineage Molecular Biology Neurons Epidermal Growth Factor Stem Cells Neurogenesis Cell Differentiation Cell Biology Neural stem cell Cell biology Neuroepithelial cell Autocrine Communication Microscopy Electron Oligodendroglia P19 cell Fibroblast Growth Factor 2 Stem cell Neuroglia Biomarkers Cell Division Adult stem cell |
| Zdroj: | Molecular and cellular neurosciences. 24(1) |
| ISSN: | 1044-7431 |
| Popis: | NTERA2 cells are a human neural cell line generating neurons after exposure to retinoic acid and, as such, are widely used as a model of neurogenesis. We report that these cells form spheres when grown in serum-free medium supplemented with basic fibroblast growth factor (bFGF) and epidermal growth factor (EGF). These spheres were found to express markers of radial glial cells such as, Pax6, glutamate transporter (GLAST), tenascin C, brain lipid-binding protein (BLBP), and the 3CB2 antigen. On plating on an adhesive substrate, NTERA2 spheres generate a large percentage of immature neurons (30-50%) together with a minority of cells of the oligodendrocyte lineage. Thus NTERA2 cells share properties with neural stem cells. However, at variance with the latter, we found that they produce their own bFGF implicated in an autocrine or paracrine proliferative loop and that they do not generate astrocytes after differentiation. These results provide an interesting model to study radial glial cells and their role in human neurogenesis. |
| Databáze: | OpenAIRE |
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