A protocol for manual segmentation of medial temporal lobe subregions in 7 Tesla MRI
| Autor: | Anica Luther, Anne Maass, Paul A. Yushkevich, Emrah Düzel, David Berron, Sandhitsu R. Das, Long Xie, Thomas Wolbers, Laura E.M. Wisse, John Pluta, Paula Vieweg, David A. Wolk, Songlin Ding, A. Hochkeppler |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Male physiology [Hippocampus] Hippocampus AG Ambient Gyrus lcsh:RC346-429 methods [Brain Mapping] 0302 clinical medicine methods [Magnetic Resonance Imaging] Cortex (anatomy) physiology [Dentate Gyrus] Perirhinal cortex diagnostic imaging [Dentate Gyrus] CSa anterior diagnostic imaging [Hippocampus] Brain Mapping medicine.diagnostic_test HB Hippocampal Body Subiculum OTS Occipito-temporal Sulcus Regular Article Magnetic Resonance Imaging Temporal Lobe CSp posterior medicine.anatomical_structure standards [Brain Mapping] Neurology PhG Parahippocampal Gyrus lcsh:R858-859.7 Female physiology [Temporal Lobe] Psychology Adult DG Dentate Gyrus PrC Perirhinal Cortex Cognitive Neuroscience CA3 Cornu Ammonis 3 lcsh:Computer applications to medicine. Medical informatics Temporal lobe 03 medical and health sciences Young Adult Neuroimaging CA1 Cornu Ammonis 1 medicine Humans Radiology Nuclear Medicine and imaging HH Hippocampal Head ddc:610 CA2 Cornu Ammonis 2 lcsh:Neurology. Diseases of the nervous system SaS Semiannular Sulcus standards [Magnetic Resonance Imaging] PhC Parahippocampal Cortex HT Hippocampal Tail Magnetic resonance imaging MTL Medial Temporal Lobe Entorhinal cortex ErC Entorhinal Cortex FG Fusiform Gyrus 030104 developmental biology diagnostic imaging [Temporal Lobe] Sub Subiculum SRLM Stratum radiatum lacunosum-moleculare Dentate Gyrus Neurology (clinical) CaS Calcarine sulcus CS Collateral Sulcus Neuroscience 030217 neurology & neurosurgery CSF Cerebrospinal Fluid |
| Zdroj: | NeuroImage : Clinical NeuroImage: Clinical 15, 466-482 (2017). doi:10.1016/j.nicl.2017.05.022 NeuroImage: Clinical, Vol 15, Iss, Pp 466-482 (2017) |
| ISSN: | 2213-1582 |
| Popis: | Recent advances in MRI and increasing knowledge on the characterization and anatomical variability of medial temporal lobe (MTL) anatomy have paved the way for more specific subdivisions of the MTL in humans. In addition, recent studies suggest that early changes in many neurodegenerative and neuropsychiatric diseases are better detected in smaller subregions of the MTL rather than with whole structure analyses. Here, we developed a new protocol using 7 Tesla (T) MRI incorporating novel anatomical findings for the manual segmentation of entorhinal cortex (ErC), perirhinal cortex (PrC; divided into area 35 and 36), parahippocampal cortex (PhC), and hippocampus; which includes the subfields subiculum (Sub), CA1, CA2, as well as CA3 and dentate gyrus (DG) which are separated by the endfolial pathway covering most of the long axis of the hippocampus. We provide detailed instructions alongside slice-by-slice segmentations to ease learning for the untrained but also more experienced raters. Twenty-two subjects were scanned (19–32 yrs, mean age = 26 years, 12 females) with a turbo spin echo (TSE) T2-weighted MRI sequence with high-resolution oblique coronal slices oriented orthogonal to the long axis of the hippocampus (in-plane resolution 0.44 × 0.44 mm2) and 1.0 mm slice thickness. The scans were manually delineated by two experienced raters, to assess intra- and inter-rater reliability. The Dice Similarity Index (DSI) was above 0.78 for all regions and the Intraclass Correlation Coefficients (ICC) were between 0.76 to 0.99 both for intra- and inter-rater reliability. In conclusion, this study presents a fine-grained and comprehensive segmentation protocol for MTL structures at 7 T MRI that closely follows recent knowledge from anatomical studies. More specific subdivisions (e.g. area 35 and 36 in PrC, and the separation of DG and CA3) may pave the way for more precise delineations thereby enabling the detection of early volumetric changes in dementia and neuropsychiatric diseases. Highlights • We present a new segmentation protocol for medial temporal lobe subregions at 7 T MRI. • Novel neuroanatomical knowledge from the work of Ding and Van Hoesen is incorporated. • Individual anatomical variability is covered by digitation- and depth-specific rules. • We provide detailed descriptions and slice-by-slice plots to guide the novice rater. • Reliability analyses provide compelling evidence that the rules can be replicated. |
| Databáze: | OpenAIRE |
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