Controlled release of moxifloxacin from intraocular lenses modified by Ar plasma-assisted grafting with AMPS or SBMA: An in vitro study
| Autor: | Benilde Saramago, Andreia F.R. Pimenta, Dimitriya Bozukova, Rogério Colaço, Ana Paula Serro, A.P. Vieira, Tiago Ruivo Correia, Maria H. Gil, A.J. Guiomar, Patrícia Alves, Ilídio J. Correia, Patrícia Coimbra |
|---|---|
| Přispěvatelé: | uBibliorum |
| Rok vydání: | 2017 |
| Předmět: |
Plasma Gases
Polymers Surface Properties Moxifloxacin 02 engineering and technology Intraocular lenses 03 medical and health sciences 0302 clinical medicine Colloid and Surface Chemistry Endophthalmitis Plasma grafting Staphylococcus epidermidis medicine Controlled release Animals Physical and Theoretical Chemistry Argon Ocular drug delivery Lenses Intraocular Chromatography biology Aqueous humour Chemistry Swelling capacity Surfaces and Interfaces General Medicine 021001 nanoscience & nanotechnology biology.organism_classification medicine.disease Anti-Bacterial Agents Drug delivery 030221 ophthalmology & optometry Microscopy Electron Scanning Surface modification Rabbits 0210 nano-technology Biotechnology medicine.drug Fluoroquinolones |
| Zdroj: | Repositório Científico de Acesso Aberto de Portugal (Repositórios Cientìficos) Agência para a Sociedade do Conhecimento (UMIC)-FCT-Sociedade da Informação instacron:RCAAP |
| ISSN: | 1873-4367 |
| Popis: | Intraocular lenses (IOLs) present an alternative for extended, local drug delivery in the prevention of post-operative acute endophthalmitis. In the present work, we modified the surface of a hydrophilic acrylic material, used for manufacturing of IOLs, through plasma-assisted grafting copolymerization of 2-acrylamido-2-methylpropane sulfonic acid (AMPS) or [2-(methacryloyloxy)ethyl]dimethyl-(3-sulfopropyl)ammonium hydroxide (SBMA), with the aim of achieving a controlled and effective drug release. The material was loaded with moxifloxacin (MFX), a commonly used antibiotic for endophthalmitis prevention. The characterization of the modified material showed that relevant properties, like swelling capacity, wettability, refractive index and transmittance, were not affected by the surface modification. Concerning the drug release profiles, the most promising result was obtained when AMPS grafting was done in the presence of MFX. This modification led to a higher amount of drug being released for a longer period of time, which is a requirement for the prevention of endophthalmitis. The material was found to be non-cytotoxic for rabbit corneal endothelial cells. In a second step, prototype IOLs were modified with AMPS and loaded with MFX as previously and, after sterilization and storage (30 days), they were tested under dynamic conditions, in a microfluidic cell with volume and renovation rate similar to the eye aqueous humour. MFX solutions collected in this assay were tested against Staphylococcus aureus and Staphylococcus epidermidis and the released antibiotic proved to be effective against both bacteria until the 12th day of release. |
| Databáze: | OpenAIRE |
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